Rabbit Anti- AP/ALP
( Alkaline phosphatase )
Catalog Number : RS-1535R
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Specificity : ·anti- AP/ALP is a rabbit polyclonal antibody unconjugated
·specific for AP/ALP of human, mouse, rat, bovine, dog
· epitope mapping near the AP/ALP of human, mouse, bovine, dog rat
origin
· use for western blotting, elisa, immunoprecipitation
and immunohistochemisty
· affinity chromatography purification, purity :>95%
· Isotype: IgG
·mol wt:58kDa
Application : ·Western blotting 1:100-500
·Immunohistochemistry 1:100-500
·ELISA 1:500-1000
·Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.