Rabbit Anti-NOTCH3
（Neurogenic locus notch homolog protein 1）
Catalog Number : RS-1812R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain （NICD） it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. It affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy （CADASIL） （swissprot）.
Specificity : · anti-NOTCH3 is a rabbit polyclonal antibody unconjugated
· specific for NOTCH3 of human, mo, rat, horse
· epitope mapping near the amino terminus of NOTCH3 of human, mo,
rat, horse origin
·use for western blotting, elisa, immunoprecipitation and
immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt:255kDa
Application : ·Western blotting 1:100-500
·Immunohistochemistry 1:100-500
·ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in
human, therapeutic or diagnostic applications.
