人單核細(xì)胞趨化蛋白4（MCP-4CCL13）檢測(cè)試劑盒齊一生物科技有限公司主要經(jīng)營進(jìn)口原裝Elisa試劑盒,種類齊全,價(jià)格實(shí)惠,貨期短.購買我公司產(chǎn)品新老客戶,可享全程技術(shù)指導(dǎo)：(包括售前標(biāo)本收集、后期實(shí)驗(yàn)、實(shí)驗(yàn)結(jié)束后數(shù)據(jù)分析等技術(shù)問題

人單核細(xì)胞趨化蛋白4（MCP-4CCL13）檢測(cè)試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的*標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長(zhǎng)處測(cè)定各孔的OD值.
MA17311    Parainfluenza Virus 1    100 μg
MA17312    Parainfluenza Virus 1    100 μg
MA17313    Parainfluenza Virus 1    100 μg
MA17314    Parainfluenza Virus 1 (FITC)    100 μg
MA17315    Parainfluenza Virus 2 (FITC)    100 μg
MA17316    Parainfluenza Virus 3    100 μg
MA17317    Parainfluenza Virus 3    100 μg
MA17318    Parainfluenza Virus 3    100 μg
MA17319    Parainfluenza Virus 3 (FITC)    100 μg
MA17321    Influenza A NP (FITC)    100 μg
MA17322    Influenza A NP (FITC)    100 μg
MA17323    Pierce(R) Anti-Influenza A NP Mouse Monoclonal Antibody,    100 μg
MA17324    Influenza A NP    100 μg
MA17325    Influenza A NP    100 μg
MA17326    Adenovirus hexon    100 μg
MA17327    Adenovirus hexon    100 μg
MA17328    Adenovirus hexon    100 μg
MA17329    Adenovirus hexon (FITC)    100 μg
MA17330    Adenovirus hexon (FITC)    100 μg
MA17331    Adenovirus hexon    100 μg
MA17332    Adenovirus hexon    100 μg
MA17333    Adenovirus hexon    100 μg
MA17334    Chlamydia trachomatis MOMP    100 μg
MA17335    Chlamydia trachomatis MOMP (FITC)    100 μg
MA17336    Chlamydia trachomatis    100 μg
MA17337    Chlamydia trachomatis (FITC)    100 μg
MA17338    Chlamydia sp. LPS    100 μg
MA17339    Pierce(R) Anti-Chlamydia sp.  LPS (FITC) Monoclonal Mouse    100 μg
MA17340    Chlamydia sp. LPS    100 μg
MA17342    Hepatitis C NS5b Ag.    100 μg
MA17343    Hepatitis C NS5b Ag.    100 μg
MA17344    Hepatitis C NS5a Ag.    100 μg
MA17345    Hepatitis C NS3 Ag.    100 μg
MA17346    Hepatitis B Surface Ag.    100 μg
MA17347    Hepatitis B Surface Ag.    100 μg
MA17348    Hepatitis B Surface Ag.    100 μg
MA17349    Hepatitis B Surface Ag.    100 μg
MA17350    Hepatitis B Surface Ag.    100 μg
MA17354    Hepatitis C NS3 Ag.    100 μg
MA17355    Hepatitis C NS3 Ag.    100 μg
MA17356    Hepatitis C Core Ag.    100 μg
MA17357    Hepatitis C NS3 Ag.    100 μg
MA17358    Hepatitis C NS4b Ag.    100 μg
MA17359    Hepatitis C NS3 Ag.    100 μg
MA17360    Hepatitis C Core Ag.    100 μg
MA17363    Hepatitis C NS4a/b Ag.    100 μg
MA17364    Hepatitis C NS4a Ag.    100 μg
MA17365    Hepatitis C NS4b Ag.    100 μg
MA17366    Hepatitis C Core Ag.    100 μg
MA17367    Hepatitis C E2 Ag.    100 μg
MA17368    Hepatitis C NS5a Ag.    100 μg
MA17369    Hepatitis C NS3 Ag.    100 μg
MA17370    Hepatitis C E1 Ag.    100 μg
MA17371    Hepatitis A Vp3    100 μg
MA17374    Hepatitis B E Ag.    100 μg
MA17376    Hepatitis B E Ag.    100 μg
MA17377    Hepatitis B Pre-S    100 μg
Human GTPase activating protein,GAP ELISA Kit
Human Apolipoprotein H,apo-H ELISA Kit
Human glycophosphatidylinositol,GPI ELISA Kit
Human pokeweed mitogen,PWM ELISA Kit
Human Lipopolysaccharides,LPS ELISA Kit
Human preptin ELISA Kit
Human peroxisome proliferators activator receptors alpha,PPAR-α ELISA Kit
Human Osteogenic Growth Peptide,OGP ELISA Kit
Human osteoclast differentiation factor,ODF ELISA Kit
Human Hydroxy-Pyridinium crosslinks,OH-PYD ELISA Kit
Human Deoxypyridinoline crosslinks,DPD ELISA Kit
Human Pyridinoline crosslinks,PYD ELISA Kit
Human Staphylococal protein A,SPA ELISA Kit
Human concanavalin A,ConA ELISA Kit
Human Free erythrocyte proloporphyrin,FEP ELISA Kit
Human lipolysaccharide binding protein,LBP ELISA Kit
Human Glutathione peroxidase,GSH-Px ELISA Kit
Human glutathione,GSH ELISA Kit
Human 17-ketosteroids,17-KS ELISA Kit
Human 17-Hydroxycorticosteroids,17-OHCS ELISA Kit
Human Cathepsin K,cath-K ELISA Kit
Human bone morphogenetic proteins,BMPs ELISA Kit
Human proteolipid protein,PLP ELISA Kit
Human vanillylmandelic acid,VMA ELISA Kit
Human glucokinase regulatory protein,GKRP ELISA Kit
Human hemagglutinin,HA ELISA Kit
Human adipose differentiation-related protein,ADRP ELISA Kit
Human Caldesmon,CALD ELISA Kit
Human calcineurin,CaN ELISA Kit
Human CTX-2 ELISA Kit
Human Calretinin,CR ELISA Kit
Human Bone Morphogenetic Protein 6,BMP-6 ELISA Kit
Human fatty acid-binding protein,FABP ELISA Kit
Human ATP-binding cassette transporter A1,ABCA1 ELISA Kit
Human Hydroxyproline-Rich Glycoprotein,HRGP ELISA Kit
Human Glucose transporter 1,GLUT1 ELISA Kit
Human low density lipoprotein,LDL ELISA Kit
Human Arachidonic Acid,AA ELISA Kit
Human bone sialoprotein,BSP ELISA Kit
Human bone alkaline phosphatase,BALP ELISA Kit
Human Cyclophosphamide,CTX ELISA Kit
Human 1,3-Disphosphoglycerate,1,3-DPG ELISA Kit
Human 1,5-anhydroglucitol,1,5-AG ELISA Kit
Human Beta-Hydroxybutyric Acid,β-OHB ELISA Kit