糖基化依賴的細(xì)胞黏附分子（GlyCAM-1）檢測(cè)試劑盒Human glycosylation-dependent cell adhesion molecule-1,GlyCAM-1 ELISA Kit全國：歡迎選購！,或咨詢?cè)诰€客服,或者以郵件形式發(fā)到我司741653262，公司提供*進(jìn)口，國產(chǎn)elisa試劑盒，均為高性價(jià)比試劑盒

糖基化依賴的細(xì)胞黏附分子（GlyCAM-1）檢測(cè)試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的*標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長處測(cè)定各孔的OD值.
9020979    Multi.Carrier (2) for 12 cult.tubes 15ml    
9020910    Cable, HEPA filter, SMC, QGX    
9020714    Cable, mains harness, C34    
9020636    Panel, front, Cabinet Qsym    
9020624    Tool, LED spot size insert, RG    
9020319    PM kit, C12 & QIAgility (UV), CG1    
9017625    Motor, y-drive (24V), BR8RD    
9020254    LED, LightStrip (white), Qsym    
9017354    Holder, VacTrap, BR8    
9020201    PCB, lrge breakout, 12 core to rev8, CC2    
9020199    PCB, Z plate breakout,8 ch., 8 core, QX1    
9020193    PCB, vac pump, QXT    
9239523    Fitting,1/16MNPT,1/8, forWasteBottle(5)    
9239536    Assy,Terminated,RedLED,SDS (2)    
9239529    Fuse&Holder,1Amp,Fblo,PicoSmd(5)    
9020159    Mask, protection - chamber bottom, RG3    
9020145    QIAplex MDD HPV Genotyping LQ Software    
9020114    USB cable, QGX    
9020102    Packaging, TLyser2    
9020100    Shipping Lock Screw, TLyser 2    
9020094    Left Ratchet Holder, TLyser 2    
9020090    Motor 24V DC, TLyser 2    
9239518    Tubing. Peek, 1/6OD, 010ID, 10 (qty 5)    
9239492    Hyd Arm Optic Switch    
9239449    3A, 250V Fast Acting Fuse    
9232867    Sensor, pressure, 1600-1mbar    
9236775    Sealing ring, auto vac, SBS top, BR3000    
9236509    BR 8000, PM Service    The Overhaul covers all travel and labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked and inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.
9239360    Z-Axis Coupling, EZ1    
9236478    BR 9604, Full Agreement    The Full Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9236476    #Agreement, 9604, 2PM, 1 yr    An agreement for preventive maintenance provides coverage for travel, labor, and parts, plus the cost of consumables required for two maintenance visits during 1 year. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.
9232726    Block, 2-way, v1.02    
9239540    Cable Assy Extender, 2 wire, 22AWG, 12in    
9239396    Upgr. LiquiChip Analyzer (RUO)    
9239496    EX XY Motor Driver w/Encoder    
9239494    Sheath Fluid Tubing w/DeBubbler    
9239487    EX Analog PCBA    
9236515    Centrifuge, PM Service    "
Service response time (repair): not applicable
Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage"
9232888    Camera, bar-code, BL180, P3xxx/BR3000    
9236636    Cover, Wash Station, BR8 RD    
9236451    Travel Charge, additional, 1 day    
9232725    Fitting, pumping tube, black    
9232712    Label, BioRobot 9604    
9236429    Sensor, plate switch, TWI    
9239142    Installation, BR MDx DSP    
9239070    Dilutor, Kloehn Versa3    
9232568    Power Cord, USA    
9238970    Target Prep, Premium Agreement    The Premium Cover Agreement TargetPrep provides full service coverage for the BioRobot TargetPrep. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).
9238791    Angle rotor for 8 Falcon tubes 50 ml    
9232328    Cable, power PCON98 board, BR8000    
9232188    T piece, pressure relief valve, dispense    
9238906    Upper casing, BS96    
9231863    Elution adapter, auto vac, BR8000    
9238681    Service manual Zent3, german    
9238680    Grease, load-bearing bolts    This is the grease for the Sigma centrifuge. Former cat no 2000770.
9231466    Spacer, DT-Adapter, BR8    
9014582    QIAVac Multiwell Base    
9022195    Reagent Chamber Lid, PyroQ24    
9022022    Sensor, Z-axis, with cable, QXA    
9021653    Kit, stickers, eluate drawer, Qsym    
9021623    Gateway 2, CC-Link, DCU    
Pierce    MA170024    CD44    250 ug
Pierce    MA170025    CD48    250 ug
Pierce    MA170028    TCR V65 gamma/delta    250 ug
Pierce    MA170029    TCRR73 alpha/beta    250 ug
Pierce    MA17003    Bacillus anthracis Protective Ag    1 ml
Pierce    MA170030    CD26    250 ug
Pierce    MA170032    Crry    250 ug
Pierce    MA170033    CD71    250 ug
Pierce    MA170034    Myeloid Lineage    250 ug
Pierce    MA170035    CD200/OX-2    250 ug
Pierce    MA170036    Dendritic cells    250 ug
Pierce    MA170037    CD62L    250 ug
Pierce    MA170039    CD152    250 ug
Pierce    MA17004    Bacillus anthracis Spore Ag    1 ml
Pierce    MA170040    CD8    250 ug
Pierce    MA170041    CD8    250 ug
Pierce    MA170045    TCR alpha/beta    250 ug
Pierce    MA170049    CD3 zeta    250 ug
Pierce    MA17005    Bordela pertussis    1 ml
Pierce    MA170050    DAF    250 ug
Pierce    MA170053    Complement C3b    250 ug
Pierce    MA170054    Pierce(R) Anti-Complement C3b Mouse Monoclonal Antibody,    250 ug
Pierce    MA170056    Complement factor I    250 ug
Pierce    MA170057    Complement factor H    250 ug
Pierce    MA170058    CD59    250 ug
Pierce    MA17006    Borrelia burgdorferi    1 ml
Pierce    MA170060    Complement C5a Receptor    250 ug
Pierce    MA170062    CD90    250 ug
Pierce    MA170063    CD90    250 ug
Pierce    MA170064    I-Ab    250 ug
Pierce    MA170065    I-Ab    250 ug
Pierce    MA170066    I-Ek    250 ug
Pierce    MA170068    Pierce(R) Anti-I-AK Mouse     Monoclonal Antibody, 250 ug.    250 ug
Pierce    MA170071    CD5    250 ug
Pierce    MA170072    CD5    250 ug
Pierce    MA170074    CD62L    250 ug
Pierce    MA170075    Integrin alpha-4    250 ug
Pierce    MA170076    CD8    250 ug
Pierce    MA170077    CD8    250 ug
Pierce    MA170078    Erythroid cells    250 ug
Pierce    MA170079    CD117    250 ug
Pierce    MA170080    CD44    250 ug
Pierce    MA170081    Pierce(R) Anti-CD45RB Rat     Monoclonal Antibody, 250 ug    250 ug
Pierce    MA170082    LY6A/E    200 ug
Pierce    MA170084    Plaet    250 ug
Pierce    MA170087    CD134    250 ug
Pierce    MA170088    CD147    250 ug
Pierce    MA170089    Pierce(R) GITR Monoclonal Rat,250 ug    250 ug
Pierce    MA17009    Candida albicans    1 ml