齊一生物金屬硫蛋白(MT)ELISA檢測試劑盒齊一生物科技（上海）有限公司試劑種類眾多：覆蓋分析化學(xué)品,無機化學(xué)品,有機化學(xué)品,生物化學(xué)品,材料化學(xué)品.試劑信息齊全：包含試劑特性描述,詳細質(zhì)量信息,應(yīng)用方法、標(biāo)準(zhǔn),用途以及正在開發(fā)的前沿應(yīng)用.齊一生物科技（上海）有限公司：021- 60348496

齊一生物金屬硫蛋白(MT)ELISA檢測試劑盒有效期：6個月
預(yù)期應(yīng)用：ELISA法定量測定血清、血漿、細胞培養(yǎng)上清或其它相關(guān)生物液體中的含量.
說明
1．試劑盒保存：-20℃（較長時間不用時）；2-8℃（頻繁使用時）.
2．濃洗滌液低溫保存會有鹽析出,稀釋時可在水浴中加溫助溶.
3．中、英文說明書可能會有不*之處,請以英文說明書為準(zhǔn).
4．剛開啟的酶聯(lián)板孔中可能會含有少許水樣物質(zhì),此為正?，F(xiàn)象,不會對實驗結(jié)果造成任何影響.
實驗原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標(biāo)抗體的微孔中依次加入標(biāo)本或標(biāo)準(zhǔn)品、*化的抗該指標(biāo)抗體、HRP標(biāo)記的親和素,經(jīng)過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成zui終的黃色.顏色的深淺和樣品中的該指標(biāo)呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,計算樣品濃度.
試劑盒組成及試劑配制
1.         酶聯(lián)板（Assay plate ）：一塊（96孔）.
2.         標(biāo)準(zhǔn)品（Standard）：2瓶（凍干品）.
3.         樣品稀釋液（Sample Diluent）：1×20ml/瓶.
4.         *標(biāo)記抗體稀釋液（Biotin-antibody Diluent）：1×10ml/瓶.
5.         辣根過氧化物酶標(biāo)記親和素稀釋液 （HRP-avidin Diluent）：1×10ml/瓶.
6.         *標(biāo)記抗體（Biotin-antibody）：1×120μl/瓶（1：100）
7.         辣根過氧化物酶標(biāo)記親和素（HRP-avidin）：1×120μl/瓶（1：100）
8.         底物溶液（TMB Substrate）：1×10ml/瓶.
9.         濃洗滌液（Wash Buffer）：1×20ml/瓶,使用時每瓶用蒸餾水稀釋25倍.
10.     終止液（Stop Solution）：1×10ml/瓶（2N H2SO4）.
10、標(biāo)本的采集及保存
1.         血清：全血標(biāo)本請于室溫放置2小時或4℃隔夜后于1000 x g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融.
2.         血漿：可用EDTA或肝素作為抗凝劑,標(biāo)本采集后30分鐘內(nèi)于2 - 8° C 1000 x g離心15分鐘,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融.
3.         細胞培養(yǎng)物上清或其它生物標(biāo)本：1000 x g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融.
注：標(biāo)本溶血會影響zui后檢測結(jié)果,因此溶血標(biāo)本不宜進行此項檢測.
381545    Investigator ESSplex SE Plus Kit (100)
206674    Type-it CNV SYBR Green PCR +qC Kit (400)
10223    QIAGEN Genomic-tip 20/G    25 columns
27104    QIAprep Spin Miniprep Kit (50)    For 50 high-purity plasmid minipreps: 50 QIAprep Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)
12662    HiSpeed Plasmid Maxi Kit (10)    10 HiSpeed Maxi Tips, 10 QIAfilter Maxi Cartridges, 10 QIAprecipitator Maxi Modules plus Syringes, Reagents, Buffers
120024    TurboFilter 96 Plates (24)
19570    Tape Pads (5)    Adhesive tape sheets for sealing multiwell plates and blocks: 25 sheets per pad, 5 pads per pack
19781    QIAfilter Mega/Giga Cartridges (5)    5 QIAfilter Mega-Giga Cartridges, Buffer FWB
30450    Ni-NTA Superflow (500 ml)    500 ml nickel-charged resin (max. pressure: 140 psi)
34660    Penta·His Antibody, BSA-free (100 μg)    100 ug mouse anti-(H)5 (lyophilized, BSA-free, for 1000 ml working solution)
34670    Tetra·His Antibody, BSA-free (100 μg)    100 ug mouse anti-(H)4 (lyophilized, BSA-free, for 1000 ml working solution)
9017749    Tube Holder, STS MDx, 2ml (12)
9017848    Rotor, centrifuge, QIAC
9239088    User Manual, BioSprint 96
9240505    PC-104, AP196
9240631    ZLV Power Supply, 24VDC 2.4 AMP, AP96
9017846    Shaker Adapter, 2.0 ml, screw cap tubes
9240646    External BCR, 2D, HHP, AP
9240665    Nut, Flangeless,1/4-28, White, AP
9240773    Assy, Shaker, AP96
9018124    Extractor, Qsym
9018143    Shaker, Qsym
9018148    Locking, drawer, Qsym
9240958    Gripper, handler, Qsym
9018543    Anti-corrosion oil (rotor), QIAC
978801    PyroMark OneStep RT-PCR Kit (50)
9021984    Transport lock, QXA
9021892    Bracket, optical sensor, angled, QIAC
9238685    Mains switch ETA, 10 A
9238694    Temperature switch, fan control
9238708    Arrow, direction of rotation
9238788    Angle rotor 6 x 80 / 85 ml
9238797    Bucket + Plateholder,up to 3 microplates
9238821    Rect.  carrier for 2 Falcon tubes 50 ml
9015083    Light Barrier, X-Dri., SamTrack, BR8 RD
990890    TissueRuptor Disposable Probes (25)
9017341    TissueRuptor Steel Probe
9016300    Board, X-Main, Immucor, P3141 SI
9016983    Capillary Set, MultiFlow, BR8 (8)
151023    REPLI-g Mitochondrial DNA Kit (25)    DNA Polymerase, Buffers, and Reagents for 25 x 50 μl Mitochondrial DNA specific whole genome amplification reactions
129117    Nuclease-Free Water (5 Liter)
19093    Deparaffinization Solution (16ml)
337843    qBiomarker SYBR ROX FAST Mastermix (24)
9021146    Bottle, Liquid Waste, DCU
9016203    Fitting, PCV Tubing, M6x1, 4.75mm (10)
9019886    Lid, label 4CH, RG3
9019007    Magnet unit, w reflector, EZ1xl
9019009    Plunger unit, EZ1xl
9019723    Transport lock, EZ1xl
9019728    Board, motor control, Y, V2, QX
9019734    Sensor, Z, with cable, V2, QX
9019756    Valve, solenoid, V2, QX
9019935    Cable harness, X-axis 8 core, CQX
齊一生物金屬硫蛋白(MT)ELISA檢測試劑盒950280    VX Carrier RNA (5 x 1 mg)
9021032    Bracket, transfer module, Qsym
77064    exoRNeasy Serum/Plasma Maxi Kit (50)
285907    bactotype MAP PCR Kit (480)
337041    qBiomarker Array PreAMP Mix
36915    96-Well Magnet    Magnet for separating magnetic beads in wells of 96-well plates, 2 x 96-Well Microplates FB
75142    RNeasy Midi Kit (10)    10 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents and Buffers
76106    RNAlater (250ml)    250 ml RNAlater RNA Stabilization Reagent for stabilization of RNA in 125 x 200 mg tissue samples
79000    Oligotex Suspension (0.5)    0.5 ml for mRNA isolation from up to 8 mg of total RNA
203205    HotStarTaq DNA Polymerase (1000)    "4 x 250 units HotStarTaq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

FOR MORE INFO ABOUT THIS PRODUCT PLEASE SEE HOTSTARTAQ DNA POLYMERASE 250 UNITS (203203)"
210210    QIAGEN OneStep RT-PCR Kit (25)    For 25 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, 5x QIAGEN OneStep RT-PCR Buffer,* dNTP Mix,** 5x Q-Solution, RNase-free water
301307    SuperFect Transfection Reagent(4x1.2 ml)    For 160 transfections in 60 mm dishes or 640 transfections in 12-well plates
28206    MinElute Reaction Cleanup Kit (250)    250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
9015637    AC fuse, 230 V, 4A, M48
9016560    BR MDx DSP Maint.pro Plasticware Kit, V1
9239360    Z-Axis Coupling, EZ1
132605    NeXtal Stock Sodium ac. H2O (50)    1.6M Sodium acetate trihydrate - 50mL (4 x 12,5 ml tube)
9019581    Pulley, Idler Y-axis, Cxx
9019070    PyroMark Thermoplate Low, PyroQ96ID    Holder for placement of PyroMark Q96 Plate Low on heat block during annealing step prior to PyroMark Q96 ID analysis.
31310    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgG (H+L),    1 ML
31312    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgG F(ab')    1 ML
31314    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgAa, 1 mL    1 ML
31316    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgA+IgG+IgM    1 ML
31318    Pierce(R) Phosphatase Conjugated Rabbit Anti-Human IgG (Fc)    1.0 ML
31320    Pierce(R) Goat Anti-Mouse IgG,(H&L), Alkaline Phosphatase    1 ML
31321    Pierce Goat Anti-Mouse IgG    (H+L) Alkaline Phosphatase    0.2 MG
31322    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG (H+L),    1 ML
31324    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG F(ab')    1 ML
31325    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG (Fc)    1.0 ML
31326    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgMu, 1 mL    1 ML
31327    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG (Fc)    1.0 ML
31328    Pierce(R) Phosphtase Conjugated Goat Anti-Mouse IgG + IgM    1 ML
31329    Pierce(R) Phosphatase Conjuga ted Rabbit Anti-Mouse IgG (H +    1.0 ML
31330    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG+IgM (H    1 ML
31331    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG {F(a    1.0 ML
31332    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG (Fc)    1.0 ML
31333    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgM (u)    1.0 ML
31334    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG (H+L    0.5 ML
31335    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG + Ig    1.0 ML
31337    Pierce(R) Phosphatase Conjugated Rabbit Anti-Goat IgG (Fc)    1.0 ML
31340    Pierce(R) Goat Anti-Rabbit IgG, (H+L), Alkaline Phosphatase    1 ML
31341    ImmunoPure Phosphatase Conjugated Goat Anti-Rabbit IgG (Fc)    1.0 ML
31342    Pierce(R) Phosphatase Conjuga ted Goat ANti-Rabbit IgG (H+L)    1 ML
31343    Pierce(R) Phosphatase Conjugated Goat Anti-Rabbit IgG    1.0 ML
31345    ImmunoPure Phosphatase Conj. Donkey Anti-Rabbit IgG(H+L)(min    0.5 ML
31346    Pierce Goat Anti-Rabbit IgG   (H+L) Alkaline Phosphatase    0.2 MG
31350    Pierce(R) Phosphatase Conjugated Goat Anti-RatIgG (H+L), 1    1 ML
31360    Pierce(R) Phosphatase Conjugated Rabbit Anti-Sheep IgG (H+L)    1 ML
31391     Alkaline Phosphatase      20 mg 
31392     Alkaline Phosphatase      100 mg 
31400    Pierce(R) Peroxidase Conjugated Mouse Anti-Goat IgG (H+L)    1 ML
31401    Pierce(R) Peroxidase Conjugat ed Rabbit Anti-Chicken IgY (H+    1.5 ML
31402    Pierce(R) Peroxidase Conjugated Rabbit Anti-Goat IgG (H+L),    1.5 ML
31403    Pierce(R) Peroxidase Conjugated Rabbit Anti-Goat IgG {F(ab'    1.5 ML
31410    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG (H+L),    2 ML
31412    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG (H+L)(m    1.5 ML
31413    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG FC    1 ML
31414    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG F(ab')2    1.5 ML
31415    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgM (Fc5u)    2 ML
31417    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgAa, 2 ML    2 ML
31418    Pierce(R) Peroxidase Conjugated Goat Anti-Human Immunoglobul    2 ML
31420    Pierce(R) Peroxidase Conjugated Mouse Anti-Human IgG)H+L)(m    1.5 ML
31423    Pierce(R) Peroxidase Conjugated Rabbit Anti-Human IgG (Fc)    1.5 ML
31430    Pierce(R) Goat Anti-Mouse IgG,(H&L), Peroxidase Conjugated,    2 ML
31431    ImmunoPure Peroxidase         Conjugated Goat Anti-Mouse    0.2 ML
31432    Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (H+L)    1.5 ML
31433    Pierce(R) Peroxidase Conjugat ed Rabbit Anti-Goat IgG (Fc)    1.5 ML