人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA檢測試劑盒Human retinoblastoma tumor suppressor protein,pRB ELISA試劑盒齊一生物科技（上海）有限公司以真誠、主動、周到、規(guī)范、熱情的服務(wù)很快得到廣大客戶的*好評.公司秉承“適應(yīng)多樣化的需求,提供專業(yè)化的服務(wù)"的經(jīng)營理念,為客戶提供*的服務(wù).歡迎社會各界朋友前來洽談業(yè)務(wù),共創(chuàng)輝煌

人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA檢測試劑盒產(chǎn)品規(guī)格：96T/48T
產(chǎn)品庫存：現(xiàn)貨
產(chǎn)品價格：詢價
使用范圍：僅供科研檢測,不得用于臨床.
保存條件：2-8℃
發(fā)貨方式：專門快遞免費(fèi)送貨上門
產(chǎn)品價格：*,洽談！
說明書:說明書隨貨發(fā)送,您也可以直接我司在線銷售人員索取.全國：代理多種品牌進(jìn)口原裝、分裝“ELISA試劑盒".齊一生物科技有限公司作為酶聯(lián)免疫供應(yīng)商,憑借*的技術(shù),*的售前,售中,售后免費(fèi)代測服務(wù),共同鑄就高品質(zhì)的產(chǎn)品.多年專業(yè)酶免服務(wù),質(zhì)量保證,可免費(fèi)提供代測服務(wù),一周內(nèi)出結(jié)果.咨詢訂購.
ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的*標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測定結(jié)果.
在450nm波長處測定各孔的OD值.
9013004    Gripper set, T-handler
9013170    Tubing, syringe inlet 2, BR8000
9013178    Gripper set, BR8000 v 1.00
130706    NeXtal Tubes MPD Suite
130711    NeXtal Tubes MBClass Suite
990352    Filter-Tips, 1000 μl (1024)
990452    Filter-Tips, 1000 μl wide bore (1024)
9018082    LiquiChip MDD Computer
9019904    PCB - Distribution, PyroQ24
9019015    Cartridge rack, EZ1xl
9019726    Transport lock, V2, QX
9019733    Motor, Z, V2, QX
979016    Fungi ITS2 Primer 2 For Sequencing
990532    50μl Filtered Sterile Tips (960)
51192    QIAamp DNA Blood Maxi Kit (10)    For 10 DNA maxipreps: 10 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)
52304    QIAamp RNA Blood Mini Kit (50)    For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
75144    RNeasy Midi Kit (50)    50 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents and Buffers
9238479    Motor, syringe, RapidPlate
132538    NeXtal Stock Dioxane (50)    * v/v Dioxane - 50mL (5 x 10 ml tube)
132625    NeXtal Stock TRIS. HCl (50)    1M Tris(hydroxymethyl)aminomethane HCl - 50mL (4 x 12,5 ml tube)
9019573    Belt, X-axis assembly, CQx
9019598    Lead, vac trap sensor, CX1
9019603    Pins, locating, Mix and Reagent set, CG1
人視網(wǎng)膜母細(xì)胞瘤抑制蛋白(pRB)ELISA檢測試劑盒9019626    Tip ejector, Cx2
9019073    PyroMark Plate Bin, PyroQ96MD
9019653    Base Plate VPT, PyroQ96VW
970022    PyroMark Q24 CpG MLH1 (4 x 24)    For quantification of methylation level in region -209 to -188 from the transcription start site.
990391    Rack Labeling Strips
218412    Mouse miScript Primer Assay Set VXX.X
1027416    FlexiTube GeneSolution siRNA 1 nmol
9019011    Motor P, EZ1xl
9019013    Motor Z, EZ1xl
9019640    Temperature Controller, EZ1xl
9019730    Board, smart key, V2, QX
9019760    Power supply, high voltage, V2, QX
978705    PyroMark PCR Kit (800)
950240    Binding Additive (VX) (4.0 g)
950254    Wash Reagent (VXW) (650 ml)
990542    200μl Filtered Sterile Tips (960)
69990    Stainless Steel Beads, 7mm
36111    Ni-NTA Magnetic Agarose Beads (2 x 1 ml)    2 x 1 ml nickel-charged magnetic agarose beads (5% suspension)
52904    QIAamp Viral RNA Mini Kit (50)    .For 50 RNA preps: 50 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
201223    Taq PCR Core Kit (250)    "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
205213    Sensiscript RT Kit (200)    For 200 reverse-transcription reactions: Sensiscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water
12663    HiSpeed Plasmid Maxi Kit (25)    25 HiSpeed Maxi Tips, 25 QIAfilter Maxi Cartridges, 25 QIAprecipitator Maxi Modules plus Syringes, Reagents, Buffers
9238477    Tubing, OLF pump head, RapidPlate
9238990    Z-Axis Coupling Unit, EZ1
9239139    Spacer, support & pod foot, TWII
9239334    Silicone Grease, BR M48 & BR EZ1
9239346    Spotlight bulb (2), BR M48
132546    NeXtal Stock Ferric chloride (50)    1M Ferric Chloride Hexahydrate - 50mL (4 x 12,5 ml tube)
132560    NeXtal Stock Magnesium chloride (50)    2M Magnesium chloride hexahydrate - 50mL (4 x 12,5 ml tube)
132620    NeXtal Stock Tricine (50)    1M Tricine - 50mL (4 x 12,5 ml tube)
132772    NeXtal Stock Jeffamine M-600 (200)    * v/v O-(2-Aminopropyl)-O'-(2-methoxyethyl)polypropylene 500 - 200mL (20 x 10 ml tube)
9019583    Slideout electrical tray (220V), C1x
9019586    Power supply, QGX
9019601    O-ring, filter housing seal 2.5mm, CX1
9019631    Board, main assembly, HS