人大腸癌專(zhuān)一抗原3(CCSA-3)ELISA檢測(cè)試劑盒英文Human colon cancer-specific antigen-3,CCSA-3 ELISA試劑盒齊一生物科技有限公司主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒,種類(lèi)齊全,價(jià)格實(shí)惠,貨期短.購(gòu)買(mǎi)我公司產(chǎn)品新老客戶(hù),可享全程技術(shù)指導(dǎo)：(包括售前標(biāo)本收集、后期實(shí)驗(yàn)、實(shí)驗(yàn)結(jié)束后數(shù)據(jù)分析等技術(shù)問(wèn)題

人大腸癌專(zhuān)一抗原3(CCSA-3)ELISA檢測(cè)試劑盒產(chǎn)品規(guī)格：96T/48T
產(chǎn)品庫(kù)存：現(xiàn)貨
產(chǎn)品價(jià)格：詢(xún)價(jià)
使用范圍：僅供科研檢測(cè),不得用于臨床.
保存條件：2-8℃
發(fā)貨方式：專(zhuān)門(mén)快遞免費(fèi)送貨上門(mén)
產(chǎn)品價(jià)格：*,洽談！
說(shuō)明書(shū):說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線(xiàn)銷(xiāo)售人員索取.全國(guó)：代理多種品牌進(jìn)口原裝、分裝“ELISA試劑盒".齊一生物科技有限公司作為酶聯(lián)免疫供應(yīng)商,憑借*的技術(shù),*的售前,售中,售后免費(fèi)代測(cè)服務(wù),共同鑄就高品質(zhì)的產(chǎn)品.多年專(zhuān)業(yè)酶免服務(wù),質(zhì)量保證,可免費(fèi)提供代測(cè)服務(wù),一周內(nèi)出結(jié)果.咨詢(xún)訂購(gòu).
ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的*標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿(mǎn)洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿(mǎn)洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長(zhǎng)處測(cè)定各孔的OD值.
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19541    QIAvac Luer Adapter Set    For processing 1-24 QIAGEN spin columns on QIAvac 6S: 6 adapters with 4 luer connectors each, 24 plugs
19577    48-Well Blocks (24)    48-well blocks with 5 ml wells, 24 per case
9017734    Plasticware kit, IQ/OQ, EZ1
9019885    Cover, main, label Biocontrol, RG3
9019907    Switch - Vacuum VPT, PyroQ24VW
9019012    Motor Y, EZ1xl
9019025    Worktable, side wall, left, EZ1xl
9019729    Board, motor control, Z, V2, QX
9019745    EZ1 Adv. XL DNA Dried Blood Card
870501    ALK RGQ RT-PCR Kit (24)
人大腸癌專(zhuān)一抗原3(CCSA-3)ELISA檢測(cè)試劑盒36912    d12-Tube Magnet    Magnet for separating magnetic beads in 12 x 1.5 ml or 2 ml tubes
51185    QIAamp DNA Blood Midi Kit (100)    For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
68163    DNeasy Plant Maxi Kit (24)    24 DNeasy Maxi Spin Columns, 24 QIAshredder Maxi Spin Columns, RNase A, Buffers, Collection Tubes (50 ml)
79216    Buffer RLT    220 ml RNeasy Lysis Buffer
120007    Collection Microtubes and Caps    Nonsterile polypropylene tubes (1.2 ml), 2304 in packs of 96, and nonsterile polypropylene caps for Collection Microtubes
201207    Taq DNA Polymerase (5000)    "20 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
132559    NeXtal Stock Magnesium acetate (50)    1M Magnesium acetate tetrahydrate - 50mL (4 x 12,5 ml tube)
9019585    Slideout electrical tray, QGY
9019599    Housing, filter seal, CX1
9019602    Home flag, Y-axis, Cx8
9019649    Fuse kit (6.3Ax10, 5Ax5), PyroQ96
970012    PyroMark Q24 CpG p16 (4 x 24)    For quantification of methylation level in  region +148 to +174 in exon 1 of the gene.
972824    PyroMark Gold Q96 CDT Reagents (6 x 96)    For performing pyrosequencing reaction on the Q96 MD in combination with the capillary dispensing tips (CDT)
9019668    PCB - Valve, PyroQ24
950911    Capture Plate, DX (5)
981313    Rotor-Disc 100 (300)
1070498    Buffer TCL, 2x (8.5 ml),G
206624    Type-it CNV SYBR Green PCR Core Kit(400)
19055    Buffer QC    1000 ml Wash Buffer
13362    Blood & Cell Culture DNA Maxi Kit    10 QIAGEN Genomic-tip 500/G, QIAGEN Protease, Buffers
19064    Buffer N3    500 ml Neutralization Buffer
19081    Buffer AW1    242 ml Wash Buffer (1) Concentrate for 1000 spin, 250 midi, or 100 maxi preps
19530    Vacuum Regulator    For use with QIAvac manifolds
28304    QIAquick Nucleotide Removal Kit (50)    50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
9012730    Cable, flat band, X, 2300 mm
9013173    Pump, probe flush, BR8000
4510033    artus Enterovirus LC RT-PCR Kit (24) PRE
130718    NeXtal Tubes PACT Suite
19088    Reagent DX
218411    Human miScript Primer Assay Set VXX.X
9019899    Cable - Ground, PyroQ96ID
9019003    Hinge, for door, EZ1xl
9019004    Grid, UV lamp, EZ1xl
9019021    Damper, door, EZ1xl
9019759    Power supply, main, 24V, V2, QX
950154    Wash Reagent (DXW) (650 ml)
9019537    Plate Stacker A/B RD, RCS
9021031    Frame, transfer module, Qsym
337042    qBiomarker Assay PreAMP Mix
51161    QIAamp 96 DNA Blood Kit (4)    For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels
75162    RNeasy Maxi Kit (12)    12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents and Buffers
120016    QIAquick 96 Plates (24)    Individual QIAquick 96 plates and handbook. No buffers or reagents.
203443    HotStarTaq Master Mix Kit (250)    "3 x 0.85 ml HotStarTaq Master Mix* containing 250 units HotStarTaq DNA Polymerase total, 2 x 1.7 ml distilled water

- premixed solution for high PCR specificity
- HotStarTaq Master Mix, HotStarTaq DNA Polymerase, several kit sizes from 250 u up to 2500 u available"
301108    PolyFect Transfection Reagent (100 ml)    For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.
231222    QIAGEN PCR Cloning plus Kit (10)    For 10 reactions: 2x Ligation Master Mix (50 ul), pDrive Cloning Vector (0.5 ug), distilled water (1.7 ml), QIAGEN EZ Competent Cells (10 tubes, 50 ul each), SOC medium (2 x 1.9 ml)
9015638    AC fuse, 115 V, 250 8A, M48
132568    NeXtal Stock Methoxy-PEG 5000 (50)    30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)
9019577    Opto switch, Z-axis, 1 ch. , Cx2
9019594    Belt, Z-axis gearbox (black), 8 ch., Cx8
9019068    PyroMark CpG SW 1.0 (5)    "Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies. 
Five additional software licenses for PyroMark CpG SW 1.0"
9019086    PyroMark Q96 MD Software (5)    "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.

5 licenses"
9019658    O rings - VPT Fast connector, PyroVW
972012    PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16
979003    PyroMark Q96 Sample Prep Tool Cover (10)    Consumables for use with magnetic tool for isolation of single stranded DNA.
979008    PyroMark Wash Buffer (conc., 200 ml)    For washing and neutralizing the immobilized DNA.
950140    Binding Additive (DX) (4.0 g)