人二級淋巴組織趨化因子(SLC/CCL21)ELISA試劑盒英文名稱Human secondary lymphoid-tissue chemokine,SLC ELISA試劑盒本試劑盒僅供研究使用檢測范圍：歡迎或或索取原版說明書zui低檢測限：歡迎或或索取原版說明書,全國：歡迎選購！

人二級淋巴組織趨化因子(SLC/CCL21)ELISA試劑盒有效期：6個月
預(yù)期應(yīng)用：ELISA法定量測定血清、血漿、細(xì)胞培養(yǎng)上清或其它相關(guān)生物液體中的含量.
說明
1．試劑盒保存：-20℃（較長時間不用時）；2-8℃（頻繁使用時）.
2．濃洗滌液低溫保存會有鹽析出,稀釋時可在水浴中加溫助溶.
3．中、英文說明書可能會有不*之處,請以英文說明書為準(zhǔn).
4．剛開啟的酶聯(lián)板孔中可能會含有少許水樣物質(zhì),此為正?，F(xiàn)象,不會對實(shí)驗結(jié)果造成任何影響.
實(shí)驗原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標(biāo)抗體的微孔中依次加入標(biāo)本或標(biāo)準(zhǔn)品、*化的抗該指標(biāo)抗體、HRP標(biāo)記的親和素,經(jīng)過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色.顏色的深淺和樣品中的該指標(biāo)呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,計算樣品濃度.
試劑盒組成及試劑配制
1.         酶聯(lián)板（Assay plate ）：一塊（96孔）.
2.         標(biāo)準(zhǔn)品（Standard）：2瓶（凍干品）.
3.         樣品稀釋液（Sample Diluent）：1×20ml/瓶.
4.         *標(biāo)記抗體稀釋液（Biotin-antibody Diluent）：1×10ml/瓶.
5.         辣根過氧化物酶標(biāo)記親和素稀釋液 （HRP-avidin Diluent）：1×10ml/瓶.
6.         *標(biāo)記抗體（Biotin-antibody）：1×120μl/瓶（1：100）
7.         辣根過氧化物酶標(biāo)記親和素（HRP-avidin）：1×120μl/瓶（1：100）
8.         底物溶液（TMB Substrate）：1×10ml/瓶.
9.         濃洗滌液（Wash Buffer）：1×20ml/瓶,使用時每瓶用蒸餾水稀釋25倍.
10.     終止液（Stop Solution）：1×10ml/瓶（2N H2SO4）.
10、標(biāo)本的采集及保存
1.         血清：全血標(biāo)本請于室溫放置2小時或4℃隔夜后于1000 x g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融.
2.         血漿：可用EDTA或肝素作為抗凝劑,標(biāo)本采集后30分鐘內(nèi)于2 - 8° C 1000 x g離心15分鐘,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融.
3.         細(xì)胞培養(yǎng)物上清或其它生物標(biāo)本：1000 x g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融.
注：標(biāo)本溶血會影響zui后檢測結(jié)果,因此溶血標(biāo)本不宜進(jìn)行此項檢測.

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201207    Taq DNA Polymerase (5000)    "20 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
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9019585    Slideout electrical tray, QGY
9019599    Housing, filter seal, CX1
9019602    Home flag, Y-axis, Cx8
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206624    Type-it CNV SYBR Green PCR Core Kit(400)
19055    Buffer QC    1000 ml Wash Buffer
13362    Blood & Cell Culture DNA Maxi Kit    10 QIAGEN Genomic-tip 500/G, QIAGEN Protease, Buffers
19064    Buffer N3    500 ml Neutralization Buffer
19081    Buffer AW1    242 ml Wash Buffer (1) Concentrate for 1000 spin, 250 midi, or 100 maxi preps
19530    Vacuum Regulator    For use with QIAvac manifolds
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4510033    artus Enterovirus LC RT-PCR Kit (24) PRE
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9019899    Cable - Ground, PyroQ96ID
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337042    qBiomarker Assay PreAMP Mix
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75162    RNeasy Maxi Kit (12)    12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents and Buffers
120016    QIAquick 96 Plates (24)    Individual QIAquick 96 plates and handbook. No buffers or reagents.
203443    HotStarTaq Master Mix Kit (250)    "3 x 0.85 ml HotStarTaq Master Mix* containing 250 units HotStarTaq DNA Polymerase total, 2 x 1.7 ml distilled water

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301108    PolyFect Transfection Reagent (100 ml)    For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.
231222    QIAGEN PCR Cloning plus Kit (10)    For 10 reactions: 2x Ligation Master Mix (50 ul), pDrive Cloning Vector (0.5 ug), distilled water (1.7 ml), QIAGEN EZ Competent Cells (10 tubes, 50 ul each), SOC medium (2 x 1.9 ml)
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132568    NeXtal Stock Methoxy-PEG 5000 (50)    30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)
9019577    Opto switch, Z-axis, 1 ch. , Cx2
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9019068    PyroMark CpG SW 1.0 (5)    "Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies. 
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9019086    PyroMark Q96 MD Software (5)    "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.

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9019658    O rings - VPT Fast connector, PyroVW
972012    PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16
979003    PyroMark Q96 Sample Prep Tool Cover (10)    Consumables for use with magnetic tool for isolation of single stranded DNA.
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9243044    Set, brackets, transparent panel, Qsym
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Anti-β-Amyloid（1-40)  β淀粉樣肽（1-40）抗體
Anti-β-Amyloid 1-40（CT）  β淀粉樣肽 1-40 C端抗體
Anti-β-Amyloid 1-40（CT）mouse  β淀粉樣肽 1-40 C端抗體
Anti-β-Amyloid（1-42）  β淀粉樣肽（1-42）抗體
Anti-Ang-Ⅱ (Angiotensin Ⅱ)  血管緊張素II抗體
Anti-Angiotensin II receptor (AngⅡ R)  血管緊張素Ⅱ受體抗體
Anti-ANGPTL1/ANG-1 (Angiopoietin-like Protein 1)  血管位蛋白樣1/血管生成素樣蛋白-1抗體
Anti-ANGPTL2/ANG-2 (Angiopoietin-like Protein 2)  血管位蛋白樣2/血管生成素樣蛋白-2抗體
Anti-ANGPTL4/ANG-4 (Angiopoietin-like Protein 4)  血管位蛋白樣4/血管生成素樣蛋白-4抗體
Anti-Annexin A  膜粘連蛋白-A抗體
Anti-Annexin II  膜粘連蛋白-2抗體
Anti-Annexin V  膜粘連蛋白-5抗體
Anti-Annexin V (Recomb Humen Annexin V)  重組膜粘連蛋白-5(人)抗體
Anti-AP1(adaptin)  銜接蛋白1抗體
Anti-Apaf-1（Apoptosis protease activating factor-1）(CT)  凋亡蛋白活性因子-1抗體（C端）
Anti-Apaf-1（Apoptosis protease activating factor-1）(NT)  凋亡蛋白活性因子-1抗體（N端）
Anti-APC（Activated protein C）  APC活化蛋白C抗體
anti-apoD (Apolipoprotein/APOD protein )  載脂蛋白D抗體
Anti-Apo-E （Apolipoprotein E）  載脂蛋白E抗體
Anti-APP695/770（Amyloid protein precursor 695/770 ）  淀粉樣肽前體蛋白抗體
Anti-AQP-2（aquaporin Protein-2）  水通道蛋白-2抗體
Anti-AQP-3（aquaporin Protein-3）  水通道蛋白-3抗體
Anti-AQP4（aquaporin Protein-4）  水通道蛋白-4抗體
Anti-AR（Androgen Receptor)  雄激素受體抗體
Anti-ARC (ARG3.1 Activity-regulated cytoskeleton-associated protein)  ARC抗體
Anti-ARF6 (ADP-ribosylation factor 6)  ADP核糖基化因子6抗體
Anti-Aromatase P450  芳香化酶/細(xì)胞色素P450/P450抗體
anti-ARIP2a（Activin receptor 2A）  激活素受體2A/激活素受體相互作用蛋白2a抗體
anti-β-arrestin 1/2(beta-arrestin 1/2)  β-抑制蛋白1/2抗體
anti-ARIP2B（Activin receptor 2B）  激活素受體2B/激活素受體相互作用蛋白2b抗體
Anti-ART(Artemin)  膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子GDNF的一種抗體
Anti-ARα1(α1-adrenergic receptor)  α1腎上腺素能受體抗體
Anti-ASCL1/MASH1 (achaete-scute complex homolog-like protein 1)  神經(jīng)母細(xì)胞特異性轉(zhuǎn)移因子抗體
anti-ASPP1（apoptosis stimulating protein of P53 1）  p53凋亡刺激蛋白1抗體
anti-ASPP2（apoptosis stimulating protein of P53 2）  P53凋亡刺激蛋白2
Anti-AT/AGT（Angiotenogen）  血管緊張素原抗體
Anti-AT1R（Angiotensin II Type 1 Receptor）  血管緊張素Ⅱ-1型受體抗體
Anti-ATF1 (Activating Transcription Factor1)  活化復(fù)制因子1抗體
Anti-ATF2 (Activating Transcription Factor2)  活化復(fù)制因子2抗體
Anti-ATF3 (Activating Transcription Factor3)  活化轉(zhuǎn)錄因子3抗體
Anti-B7-H1/PDL1/CD274(programmed death ligand 1)  程序性死亡配體1抗體
Anti-B7-H4 ( V-set domain containing T cell activation inhibitor 1 )  B7H4抗體
Anti-BACE/ASP2   β分泌酶抗體
Anti-Bad (BCL-xL/BCL-2-associated death promoter)  相關(guān)死亡促進(jìn)因子Bad抗體
anti-Bak（BCL2 homologous antagonist/killer）  Bcl-2同源拮抗劑Bak抗體
Anti-BADH2 (Betaine-aldehyde dehydrogenase )  BADH2抗體
Anti-Bassoon/RCN(zinc finger protein)  鋅指蛋白231抗體
Anti-Bax （Bcl-2 Assaciated X protein）  Bax（小鼠來源抗體）
Anti-Bax（Bcl-2 Assaciated X protein）  Bax抗體