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更新時(shí)間:2015-10-20 09:31:28瀏覽次數(shù):319次
聯(lián)系我時(shí),請(qǐng)告知來(lái)自 環(huán)保在線NCTC clone 929 [L描述:1948年三月建立了NCTC clone 929(小鼠結(jié)締組織),細(xì)胞系L的克隆。細(xì)胞系L是Z早建立的連續(xù)培養(yǎng)細(xì)胞系之一,而clone 929是Z早的克隆株。 從一只100日齡的雄性C3H/An小鼠的正常皮下疏松結(jié)締組織入脂肪組織中建立了親本細(xì)胞系L。 第95代的細(xì)胞系L使用毛細(xì)管法分離單細(xì)胞建立了clone929。 檢測(cè)發(fā)現(xiàn)鼠痘病毒陰性。
細(xì)胞名稱 | NCTC clone 929 [L cell, L-929, derivative of Strain L]小鼠成纖維細(xì)胞 |
描述 | 1948年三月建立了NCTC clone 929(小鼠結(jié)締組織),細(xì)胞系L的克隆。細(xì)胞系L是zui早建立的連續(xù)培養(yǎng)細(xì)胞系之一,而clone 929是zui早的克隆株。 從一只100日齡的雄性C3H/An小鼠的正常皮下疏松結(jié)締組織入脂肪組織中建立了親本細(xì)胞系L。 第95代的細(xì)胞系L使用毛細(xì)管法分離單細(xì)胞建立了clone929。 檢測(cè)發(fā)現(xiàn)鼠痘病毒陰性。 |
動(dòng)物種別 | 小鼠 |
性別 | 雄 |
組織來(lái)源 | 組織:皮下結(jié)締組織;疏松結(jié)締組織及脂肪 品系:C3H/An |
形態(tài) | 成纖維細(xì)胞 |
培養(yǎng)基和添加劑 | MEM培養(yǎng)基(GIBCO,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;優(yōu)質(zhì)胎牛血清,10%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。 |
供應(yīng)限制 | A。僅供研究之用。 |
REFERENCE | 3: Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324 21373: Fisher EM, et al. Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517 21404: Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948. 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 21469: ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0813-07. 21470: ASTM International Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0895-84 (Reapproved 2006). 21606: U.S. Pharmacopeia General Chapters: <87> BIOLOGICAL REACTIVITY TESTS, IN VITRO. Rockville, MD:U.S. Pharmacopeia;USP USP28-NF23, 2005 23579: Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue and mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed: 13233820 25770: Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943. 25879: Earle WR, et al. The influence of inoculum size on proliferation in tissue cultures. J. Natl. Cancer Inst. 12: 133-153, 1951. PubMed: 14874126 25880: Sanford KK, et al. The tumor-producing capacity of strain L mouse cells after 10 years in vitro. Cancer Res. 16: 162-166, 1956. PubMed: 13293658 25882: Westfall BB, et al. The arginase and rhodanese activities of certain cell strains after long c*tion in vitro. J. Biophys. Biochem. Cytol. 4: 567-570, 1958. PubMed: 13587550 29223: Papkoff J. Regulation of complexed and free catenin pools by distinct mechanisms. J. Biol. Chem. 272: 4536-4543, 1997. PubMed: 9020180 32283: Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991 33114: Yasin B, et al. Susceptibility of Chlamydia trachomatis to protegrins and defensins. Infect. Immun. 64: 709-713, 1996. PubMed: 8641770 92346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002. 92380: Plastics collapsible containers for human blood and blood components--Part 1: Conventional containers. Annex C. Biological tests.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 3826-1:2003. 92382: Dentistry--Preclinical evaluation of biocompatibility of medical devices used in dentistry--Test methods for dental materials. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 7405:1997. 92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999. 92404: Plastic containers for intravenous injection. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 15747:2003. |
中文名稱 | 小鼠成纖維細(xì)胞 |
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