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英文名稱(chēng) ELAC2
別 名 B3Gal T8; Beta 1 3 galactosyltransferase; C1GALT; Core 1 beta1 3 galactosyltransferase 1; Core 1 beta3 Gal T; Core 1 O glycan T synthase; Core 1 synthase glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1; Core 1 UDP galactose:N acetylgalactosamine alpha R beta 1 3 galactosyltransferase; Glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1; T synthase.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 兔來(lái)源和鼠來(lái)源
克隆類(lèi)型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit
產(chǎn)品類(lèi)型 一抗
研究領(lǐng)域 心血管 細(xì)胞生物 免疫學(xué)
蛋白分子量 predicted molecular weight: 40kDa
性 狀 Lyophilized or Liquid
實(shí)驗(yàn)步驟
① 滴加0.01mol/L，pH7.4的PBS于待檢標(biāo)本片上，10min后棄去，使標(biāo)本保持一定濕度。
② 滴加適當(dāng)稀釋的熒光標(biāo)記的抗體溶液，使其*覆蓋標(biāo)本，置于有蓋搪瓷盒內(nèi)，保溫一定時(shí)間（參考：30min）。
③ 取出玻片，置玻片架上，先用0.01mol/L，pH7.4的PBS沖洗后，再按順序過(guò)0.01mol/L，pH7.4的PBS三缸浸泡，每缸3-5 min，不時(shí)振蕩。
④ 取出玻片，用濾紙吸去多余水分，但不使標(biāo)本干燥，加一滴緩沖甘油，以蓋玻片覆蓋。
⑤ 立即用熒光顯微鏡觀察。觀察標(biāo)本的特異性熒光強(qiáng)度，一般可用“+"表示：
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protein kinase, cAMP-dependent, regulatory, type II, alpha |
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plasminogen activator, tissue |
enolase 2 (gamma, neuronal) |
GNAS complex locus |
penta-EF-hand domain containing 1 |
Rho guanine exchange factor (GEF) 16 |
annexin A7 |
cell division cycle 42 (GTP binding protein, 25kDa) |
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tumor suppressor candidate 4 |
TAO kinase 3 |
v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) |
chromosome 17 open reading frame 81 |
melanin-concentrating hormone receptor 1 |
keratin 13 |
eukaryotic translation elongation factor 1 gamma |
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acyl-CoA synthetase medium-chain family member 3 |
heat shock factor binding protein 1 |
complement factor B |
transforming growth factor beta regulator 4 |
chorionic somatomammotropin hormone 1 (placental lactogen) |
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