細(xì)胞名稱

EDJ#22小鼠胚胎干細(xì)胞EDJ#22（干細(xì)胞庫(kù)保藏）

描述

小鼠*性胚胎干細(xì)胞，每支細(xì)胞量1×10⁶

動(dòng)物種別

小鼠，129S5/SvEvTac品系

性別

雄

組織來源

小鼠胚胎內(nèi)細(xì)胞團(tuán)

形態(tài)

球形克隆

培養(yǎng)基和添加劑

mES*培養(yǎng)液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES級(jí)FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（終濃度為1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999. PubMed: 10430671 Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391 Threadgill DW, et al. Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain. Mamm. Genome. 8(6):390-393 1997. PubMed: 9166580 Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865