細(xì)胞名稱

R1小鼠胚胎干細(xì)胞R1（干細(xì)胞庫保藏）

描述

小鼠*性胚胎干細(xì)胞，每支細(xì)胞量1×106

動(dòng)物種別

小鼠，129X1 × 129S1品系

性別

雄

組織來源

小鼠胚胎內(nèi)細(xì)胞團(tuán)

形態(tài)

球形克隆

培養(yǎng)基和添加劑

mES*培養(yǎng)液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES級(jí)FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（終濃度為1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach. Oxford, Oxford University Press, 101-132, 1999 Nagy A, et al. Derivation of compley cell culture-derived mice from early-passage embryonic stem cells. Proc. Natl. Acad. Sci. USA: 8424-8428, 1993. PubMed: 8378314 Wood SA, et al. Non-injection methods for the production of embryonic stem cell-embryo chimaeras. Nature 365: 87-89, 1993. PubMed: 8361547 Nagy A, Rossant J. Production and analysis of ES-cell aggregation chimeras. In: Nagy A, Rossant J. Gene Targeting: A Practical Approach. Oxford, Oxford University Press, 177-206, 1999