細(xì)胞名稱(chēng)

A-375人惡性黑色素瘤細(xì)胞（2013年新引進(jìn)）（干細(xì)胞庫(kù)保藏）

描述

人惡性黑色素瘤細(xì)胞

動(dòng)物種別

人

性別

女性

組織來(lái)源

皮膚

形態(tài)

上皮樣

培養(yǎng)基和添加劑

DMEM (Invitrogen, 12430) 89 ml 　　 FBS (Biochrom, S4115) 10 ml Sodium Pyruvate (Invitrogen, 11360-070) 1 ml（終濃度1 mM） 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Giard DJ, et al. In vitro c*tion of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Cormier JN, et al. Heterogeneous expression of melanoma-associated antigens and HLA-A2 in metastatic melanoma in vivo. Int. J. Cancer 75: 517-524, 1998. PubMed: 9466650 Sharma SD, et al. Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors. Proc. Natl. Acad. Sci. USA 93: 13715-13720, 1996. PubMed: 8943000

癌細(xì)胞

動(dòng)物種別

人

性別

女性

組織來(lái)源

卵巢

形態(tài)

上皮樣

培養(yǎng)基和添加劑

DMEM (Invitrogen, 12430) 89 ml FBS (Biochrom, S4115) 10 ml Sodium Pyruvate 100 mM Solution（invitrogen 11360070） 1 ml

供應(yīng)限制

REFERENCE

Karlan BY, et al. Glucocorticoids stabilize HER-2/neu messenger RNA in human epithelial ovarian carcinoma cells. Gyn. Onc. 53: 70-77, 1994. PubMed: 7909787