雙氯青霉素檢測(cè)試劑盒規(guī)    格：96T

檢測(cè)標(biāo)本：各種動(dòng)物肌肉組織、肝臟、尿液、血清、水產(chǎn)、蜜蜂、牛奶、糧食作物（玉米、大米、豆類(lèi)、花生、麥類(lèi)等）、飼料等

檢測(cè)方法：ELISA

檢測(cè)類(lèi)型：酶聯(lián)免疫分析法

產(chǎn)品的用途：僅供科研究課題使用 

雙氯青霉素檢測(cè)試劑盒價(jià)格及詳細(xì)資料：電議,或咨詢?cè)诰€客服,或者以郵件形式發(fā)到我司qysw@qiyibio.com .齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的*肯定和認(rèn)同。*保證，價(jià)格公道，傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供的產(chǎn)品。若有需要，我司將竭誠(chéng)為您服務(wù)！

                 本試劑盒用于測(cè)定各種動(dòng)物肌肉組織、肝臟、尿液、血清、飼料等水產(chǎn)/蜂蜜/牛奶相關(guān)液體樣本中殘留的定量檢測(cè)。 

實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平。用純化的本產(chǎn)品抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入本產(chǎn)品抗原，再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度。

試劑盒組成：

標(biāo)本要求： 

1．標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn)，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品，因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性。

操作步驟：

• 標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔，在*、第二孔中分別加標(biāo)準(zhǔn)品100μl，然后在*、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl，混勻；然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔，再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻；然后在第三孔和第四孔中先各取50μl棄掉，再各取50μl分別加到第五、第六孔中，再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul，混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中，再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻后從第七、第八孔中分別取50μl加到第九、第十孔中，再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻后從第九第十孔中各取50μl棄掉。（稀釋后各孔加樣量都為50μl，濃度分別為1200 ng/L，800 ng/L ，400 ng/L，200ng/L， 100 ng/L）。
• 加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑，其余各步操作相同）、待測(cè)樣品孔。在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl，然后再加待測(cè)樣品10μl（樣品zui終稀釋度為5倍）。加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻。
• 溫育：用封板膜封板后置37℃溫育30分鐘。
• 配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用。
• 洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復(fù)5次，拍干。
• 加酶：每孔加入酶標(biāo)試劑50μl，空白孔除外。
• 溫育：操作同3。
• 洗滌：操作同5。
• 顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘. 
• 終止：每孔加終止液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。
• 測(cè)定：以空白空調(diào)零，450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）。 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。

注意事項(xiàng)：

• 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開(kāi)封后如未用完，板條應(yīng)裝入密封袋中保存。
• 濃洗滌液可能會(huì)有結(jié)晶析出，稀釋時(shí)可在水浴中加溫助溶，洗滌時(shí)不影響結(jié)果。
• 各步加樣均應(yīng)使用加樣器，并經(jīng)常校對(duì)其準(zhǔn)確性，以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，使用排槍加樣。
• 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值），請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定，計(jì)算時(shí)請(qǐng)zui后乘以總稀釋倍數(shù)（×n×5）。
• 封板膜只限一次性使用，以避免交叉污染。
• 底物請(qǐng)避光保存。
• 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行，試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
• 所有樣品，洗滌液和各種廢棄物都應(yīng)按傳染物處理。
• 本試劑不同批號(hào)組分不得混用。

10. 如與英文說(shuō)明書(shū)有異，以英文說(shuō)明書(shū)為準(zhǔn)。

計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo)，OD值為縱坐標(biāo)，    

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線，根據(jù)樣品的OD      

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋      

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)      

準(zhǔn)曲線的直線回歸方程式，將樣品的OD值      

代入方程式，計(jì)算出樣品濃度，再乘以稀釋

倍數(shù)，即為樣品的實(shí)際濃度。

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上。

2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%     

保存條件及有效期：

1.試劑盒保存；2-8℃。

2．有效期：6個(gè)月

其它產(chǎn)品

9243772    QIAsymphony AS Custom Protocol Basic1        38,830.00     CNY
380325    Investigator Triplex DSF Kit (100)        11,310.00     CNY
9001297    QIAsymphony SP        1,050,990.00     CNY
130919    NeXtal DWBlock Nucleix Suite        3,490.00     CNY
4811014    careHCV RT-PCR ASSAY V2        3,710.00     CNY
4857021    careMP PCR ASSAY        1,930.00     CNY
130825    NeXtal CubicPhase 3w Mo/C 8% µplate (2)        1,850.00     CNY
4555205    artus M.tuberculosis RG PCR Kit (96) RUO        45,960.00     CNY
130728    Nextal Tubes CubicPhase I Suite        8,350.00     CNY
4513263    artus HI Virus-1 RG RT-PCR Kit (24) CE        11,870.00     CNY
130719    NeXtal Tubes Nucleix Suite        8,350.00     CNY
129834    GelPilot Small Fragment Agarose (500g)        24,830.00     CNY
129814    GelPilot LE Agarose (500g)        6,910.00     CNY
129046    MaXtract High Density (200 x 1.5 ml)        2,400.00     CNY
4504163    artus Parvo B19 TM PCR Kit (24) CE        11,870.00     CNY
79000    Oligotex Suspension (0.5)    0.5 ml for mRNA isolation from up to 8 mg of total RNA    5,280.00     CNY
76106    RNAlater (250ml)    250 ml RNAlater RNA Stabilization Reagent for stabilization of RNA in 125 x 200 mg tissue samples    4,050.00     CNY
75142    RNeasy Midi Kit (10)    10 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents and Buffers    1,850.00     CNY
27191    QIAprep 96 Turbo Miniprep Kit (4)    For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml), Caps    12,170.00     CNY
74004    RNeasy Micro Kit (50)    50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents and Buffers    5,870.00     CNY
19504    QIAvac 96    Vacuum manifold for processing  QIAGEN 96-well plates: includes QIAvac 96 Top Plate, Base, Waste Tray, Plate Holder, Rack of Collection Microtubes (1.2 ml)    9,090.00     CNY
19083    Buffer PM (500ml)    500 ml Binding Buffer    729.00     CNY
9015341    Board, IRB        8,940.00     CNY
133046    NeXtalStock Ferric chloride  (200)        1,870.00     CNY
9012227    Shaker, Tube rack for Plato 7        57,410.00     CNY
9012140    Gripper, right, handler, BR8000        1,000.00     CNY
9001670    PyroMark Q24 MDx Instrument    For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.    791,960.00     CNY
9001660    Rotor-Gene Q 6plex System        379,200.00     CNY
9001649    Rotor-Gene Q 5plex (w/o PC) PrioPLUS    Rotor-Gene Q 5plex (w/o PC) PrioPLUS includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 3 years with shipment to Repair Center Service and response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-compliant Field Service documents.    434,910.00     CNY
9001648    Rotor-Gene Q 5plex (w/o PC) Priority    Rotor-Gene Q 5plex (w/o PC) Priority includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-compliant Field Service documents.    402,760.00     CNY
950263    Final Wash Reagent (VXF) (350 ml)        902.00     CNY
37561    Qproteome Sialic Glycoprotein Kit        5,760.00     CNY
133029    NeXtalStock Cesium chloride (200)        2,770.00     CNY
133021    NeXtalStock CABS untit. (200)        9,640.00     CNY
9001590    Rotor-Gene Q 6plex Platform        369,170.00     CNY
239025    GelPilot 50 bp Ladder (100)    Ready-to-use DNA marker; 600 µl (100 lanes), 9 fragments: 50 - 500 bp    1,770.00     CNY
231122    QIAGEN PCR Cloning Kit (10)    For 10 reactions: 2x Ligation Master Mix (50 ul), pDrive Cloning Vector (0.5 ug), distilled water (1.7 ml)    1,200.00     CNY
9015317    Sensor, photo, EE-SX671A        647.00     CNY
9015305    Block, elution, M48        96,780.00     CNY
133148    NeXtalStock BICINE pH 7.5 (200)        2,120.00     CNY
1037870    Buffer TLC, 2x (50/60),G        1,650.00     CNY
4692103    RespiFinder RG Panel RUO        25,180.00     CNY
1031668    Large 96-Rod Cover (16)    Rod cover for use with BioSprint 96 Deep well Rod    1,470.00     CNY
9011958    SamTrak Rack for sixteen 12mm tubes        2,470.00     CNY
9243827    RCS, Basic Agreement    "- Service response time RCS: 5 working days
- Inspection/maintenance delivery: yes, included; 1 /agreement year
- Cost coverage for repair parts: yes, included
- Cost coverage for labor: yes, included
- Cost coverage for travel: yes, included
- Replacement system provision for DML: yes, included
- Transportation cost coverage for DML: yes, included
 
For defect units, after customer calling QIAGEN technical service hotline before 12 am:
 
RCS: 
- QIAGEN shall dispatch a specialist to repair the defect instrument at customer site within 5 working days
 
DML:
- A special package containing a replacement unit shall be sent to the customer within 5 working days.
- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.   

Note: for DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer and Votexer:
- A new unit shall be sent to customer within  7-10 working days at no extra cost
- Customer shall send the defect unit to QIAGEN for disposal
- On-site installation of PC and application software is not covered by the agreement
 
The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines and documented according to GLP / GMP"    87,670.00     CNY
37003    #Albumin/IgG Depletion Cartridges(3x1ml)        14,920.00     CNY
36311    Strep-Tactin Magnetic Beads (2x1ml)    For micro-scale purification of Strep-tagged proteins: 2 x 1 ml Strep-Tactin-charged magnetic agarose beads (10% suspension)    3,920.00     CNY
950183    Tissue Digest Reagent (DXT) (250 ml)        504.00     CNY
950047    QIAxtractor Virus Plasticware    The Reagent Pack, VX,  (#950407) which is used together with the Virus Plasticware Pack, QIAxtractor provides fully automated viral nucleic acid purification on the QIAxtractor. The combination of reagents and plasticware enables purification of viral nucleic acids from liquid samples, with a throughput of 8-96 samples per run.    2,860.00     CNY
218413    Rat miScript Primer Assay Set VX.X        185,230.00     CNY
9018297    EZ1 Advanced RNA Card        10,770.00     CNY
9018296    EZ1 Advanced DNA Buccal Swab Card        10,770.00     CNY
5000-1271    Y Drive Track, DML2        1,930.00     CNY
5000-1265    Mirror, DML2        8,140.00     CNY
5000-1261    PMT Socket Only, DML2        7,800.00     CNY
5050-1072 V.1    CD Rom Manual - V2 Software Tutorial I        602.00     CNY
5000-1508    Microplate Heater I - FuseInlet1.6A 250V        127.00     CNY
6000-5002    MST STM Rack (Black - 1 pos. cal.)        6,410.00     CNY
6000-4111DEV2.2    RCS User Application Manual (German)        3,410.00     CNY
6000-3111V2.20    RCS User Application Manual        2,280.00     CNY