人轉(zhuǎn)錄因子AP-1 ELISA kit

產(chǎn)品類(lèi)型:

ELISA Kit

• 產(chǎn)品名稱(chēng):

  人轉(zhuǎn)錄因子AP-1 ELISA kit

• 英文名稱(chēng):

  Human Transcription Factor/Activator Protein 1 (AP-1) ELISA kit

• 人轉(zhuǎn)錄因子AP-1 ELISA kit
• 別名:

  AP-1, AP1, c-Jun, Jun activation domain binding protein|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog

• 規(guī)格:

  96T

• 種屬:

  Human

• 待測(cè)物名稱(chēng):

  jun oncogene

• 縮寫(xiě):

  JUN

• 蛋白功能1:

  Transcription/Transcription regulation

• 蛋白功能3:

  Transcription

• 檢測(cè)范圍:

  47 pg/ml-3000 pg/ml

• 靈敏度:

  11.75 pg/ml

• 反應(yīng)時(shí)間:

  1-5h

• 所需樣本體積:

  50-100ul

• 檢測(cè)波長(zhǎng):

  450 nm

• This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.