 |
上海哈靈生物科技有限公司
主營(yíng)產(chǎn)品： ELISA代測(cè)服務(wù),ELISA試劑盒價(jià)格,ELISA試劑盒,ELISA試劑盒說(shuō)明書(shū),大鼠ELISA試劑盒 |
會(huì)員.png)
聯(lián)系電話(huà)
18918128223
公司信息
- 聯(lián)系人：
- 何經(jīng)理
- 電話(huà)：
- 手機(jī)：
- 18918128223
- 傳真：
- 86-021-51010234
- 地址：
- 國(guó)定東路200號(hào)
- 郵編：
- 200433
- 網(wǎng)址：
- www.halingbio.com/
The FGF-23 ELISA Kit
2013-9-24 閱讀(723)
The FGF-23 ELISA Kit is a two-site enzyme-linked immunosorbent assay kit for the measurement of FGF-23 in serum. Two specific murine monoclonal antibodies bind to full-length FGF-23. One antibody is immobilized onto the microtiter plate well for capture. The other antibody is conjugated to HRP（horseradish peroxidase） for detection.
In first reaction, a sample containing FGF-23 is incubated with the immobilized antibody in a microtiter well. FGF-23 in the sample is captured with the antibody. At the end of this reaction, the well is washed to remove unbound FGF-23 and other components.
In second reaction, this immobilized FGF-23 is incubated with HRP labeled antibody to form a “sandwich”complex :
Anti FGF-23 antibody - N-terminal FGF-23 C-terminal - HRP labeled anti FGF-23 antibody
At the end of this reaction, the well is washed to remove unbound components.
In enzyme reaction, the sandwich complex immobilized on the well is incubated with a substrate solution and then measured by a spectrophotometric microtiter plate reader. The enzymatic activity of the complex bound to the well is directly proportional to the amount of FGF-23 in the sample.
A standard curve is generated by plotting the absorbance versus the each concentration of FGF-23 standard. The concentration of FGF-23 in the sample is determined from this curve.
