徠卡倒置顯微鏡DMIL LED產(chǎn)品介紹：
LEICA DM IL LED – BRILLIANT OPTICS COMBINED WITH INNOVATIVE ILLUMINATION*The Leica DM IL LED is exceptionally well-suited for uses ranging from various cell and tissue culture examinations in life sciences, developmental biology studies or micromanipulation in cell biology to living cell examinations in transgenics or electro-physiology.
* The ?uorescence version, the Leica DM IL LED Fluo, also offers a variety of possible applications. Optionally, it is also available with the new LED illumination.
* Heatable microscope stages and 3-plate cross-stages provide great ? exibility for experiments on living cells under physiological conditions.
* The Leica DM IL LED has a further advantage that distinguishes it from other microscopes in its class: The stａnd is highly com-patible with components of the Leica research microscopes. Objectives, eyepieces, tubes, camera ports, contrast methods. Additionally, special tubes and condensers have been developed for the Leica DM IL LED.
Integrated ? uorescence
* Manual ? uorescence with three ? lter cubes.
* Integrated shutter.
* Optionally LED, classic mercury illumination or ?ber optic coupling
Flexible and modular
* A full range of optical components
* Compatible with research stands
* Unheated and heated stages
* Large selection of tubes
* Comprehensive range of accessories for special applications
The Most Comprehensive Array of Contrast Methods
All available contrast methods can be adapted to individual applications easily and quickly. Two condensers have been devel-oped speci? cally for the Leica DM IL LED, which can be used for the entire magni? cation range of the respective contrast method. The high-resolution S40/0.45 condenser makes even tiny details of a specimen visible. Both condensers, the S40/0.45 and S80/0.30, allow for use of phase contrast up to the 63x objective as well as Integrated Modulation Contrast (IMC) up to the 40x objective.
Fatigue-free operation
The ergonomic arrangement of all controls such as the focus dial, brightness controller, condenser height adjustment, objective nosepiece and XY stage adjustment allow users to be relaxed while working with the microscope – even for hours. The height-adjustable stages, Ergo tubes with variable tube height, ? exible viewing height, and the interpupillary distance and diopter setting enable each user to con? gure his or her personal Leica DM IL LED. The large working distances provide suf? cient room for large cul-ture ? asks, and the unobstructed view of the specimen area fa-cilitates handling more dif? cult specimens.
Bright ?eld
All Leica bright? eld and phase objectives from 2.5x to 100x can be used for stained specimens. The bright ? eld method can be used even for low magni? cation levels without condenser. A working distance of 200 mm is realized simply by unscrewing the condenser head.
Phase contrast
Phase contrast is used primarily in live cell microscopy to make structures in unstained specimens visible. Three preadjusted light rings on a slider allow phase contrast for all objectives from 5x to 63x. No readjustment is necessary when changing the objectives. The intelligent LED illumination adjusts the brightness automati-cally when switching between the phase contrast and bright? eld method.
Integrated Modulation Contrast (IMC)
IMC creates relief-type images and has proven to be an alter-native to Differential Interference Contrast (DIC), particularly in micromanipulation. The IMC developed by Leica Microsystems does not require special objectives because the IMC modulator is not integrated into the objective, but operated via a separate slider. The IMC illumination slider is encoded and controls the LED illumi-nation. IMC is available for both condensers and for standard 10x, 20x, 32x and 40x objectives.
Fluorescence
Incident-light ? uorescence is an integral part of the Leica DM IL LED Fluo microscope variant. The ? uorescence slider holds three ? lter blocks. The transmitted light method and ? uorescence can be used simultaneously. This way, object structures can be clearly assigned. Each ? lter block comprises an optimally matched combi-nation of excitation, re? ection and barrier ? lters. Illumination can be generated via the Leica SFL100 LED illumination, the classic mercury illumination or the Leica EL6000 ? ber optic coupling.
Longworking Distance Condenser & Perfectly Illuminated
For the ? rst time, a condenser concept has been realized in the Leica DM IL LED that allows all contrast methods with all con-densers. With a working distance of at least 40 mm and a numeri-cal aperture of 0.45, the S40 condenser is the perfect tool for applications for which optimum resolution is the most important parameter. Phase contrast and IMC ensure optimum contrasting.
The working distance of at least 80 mm and 0.30 aperture of the S80 condenser are the ideal prerequisites for achieving maximum possible free room around the specimen and optimum contrasting at the same time. The continuous adjustment of the condenser height depending on specimen vessel and liquid layer is a one-of-a-kind feature. It ensures maximum ? exibility when using periph-eral microtools.
Whether you work with thin sections or thick specimens, phase and modulation contrast produce a brilliant microscopic image for all specimens and applications.
Flexible Fluorescence
Fluorescence applications, in particular GFP labeling, play an ever more important role in clinical diagnostics and routine microscopy. The Leica DM IL LED Fluo has been designed in consideration of this trend. The microscope has been equipped with a ? uorescence axis and a 3-position slider to ensure fast and easy switching to different ? uorochromes. The slider glides smoothly in an elabo-rately constructed dovetail guide. An extensive, constantly grow-ing range of ? lters allows a wide variety of ? uorescence exami-nations. The ? lter blocks are optimized for minimizing stray light. Excitation, re? ection and barrier ? lters adapt to your application. Transmitted-light methods can be used simultaneously or as an alternative so that ? uorescent and non? uorescent structures can be clearly assigned. An integrated shutter protects the specimen against bleaching.
The Leica DM IL LED Fluo is the ? rst routine ? uorescence microscope that allows users to choose between classic illumination (halogen, mercury or high-pressure xenon lamps), the ”cold” light guide cou-pling of the Leica EL6000 and the new LED illumination, Leica SFL100. This gives users the ability to excite and examine ? uorochromes to see more detail under the microscope. A dark background and the bright emitted ? uorescence produce brilliant color images. The ? eld of applications ranges from DAPI (UV) for nuclear staining to CY5 (IR) for immuno-histochemical arrays. The Leica DM IL LED Fluo thus is a high-performance instrument for use in immunology, cytology, virolo-gy – and in any ?eld that requires ?uorescence techniques for living specimens.
Apart from the Ergo Modules for variable height adjustment, Leica Microsystems offers a drawing attachment for special examina-tions and a discussion attachment for two observers.
In live cell microscopy, the right microscope stage and the cor-responding accessories are important prerequisites for the best results. In addition to the ? xed stages with or without mechanical stage, Leica Microsystems offers 3-plate cross-stages with differ-ent inserts for a wide variety of culture ? asks. All microscopes are also available with heating stages or heating inserts. The sturdy mechanical system and the compact stａnd ensure best possible stability.
徠卡倒置顯微鏡DMIL LED基本參數(shù)：
| Leica DM IL LED | |
|---|---|
| Optics | In? nity corrected (HCS), tube factor 1x |
| Field of view | 20 mm |
| Lamp power supply | AC input: 100-240 V /0.33-0.19 A DC output: 5 V /2 A |
| Illumination | 5 watt LED |
| Focusing | Coarse and ? ne adjustment, nosepiece focusing, vertical travel 7 mm |
| Objective Nosepiece | 4-position, M25x0.75 objective thread |
| Stage | Fixed work stage with 3-point support 248 x 212 x 20 mm |
| Heating stage 248 x 212 x 20 mm incl. TempControl 2000 | |
| 3-plate stage, 150 x 150 mm insert plate, adjustment range 60 x 40 mm | |
| Transmitted-light illuminator arm | with illumination unit, with precentered LED illumination incl. collector, diffusion ?lter, iris aperture diaphragm, condenser holder |
| Additionally for ? uorescence versions: | Lamp housing: Interchangeable lamp housings for ? uorescence |
| Fluorescence: Integrated lamp mount in a massive, stable back panel, integrated ? uorescence axis, 3-position ? uorescence slide for three different ? lter blocks, dark stop | |
Overview of the Leica DM IL LED
| Optics | Leica HC optics (in? nity corrected) HC objectives: 2.5x–100x | DM IL LED Fluo | DM IL LED | |
| Objective Nosepiece | Four positions | ● | ● | |
| Focus | Coaxial coarse and ? ne adjustment, travel path 7 mm, nosepiece focusing | ● | ● | |
| Transmitted-light illuminator | 5 watt LED, power supply (in 100-240, out 5 V/2 A) Filter holder for TL ? lter Ø 32 mm, collector, scattering ? lter | ● | ● | |
| Condenser | Interchangeable condenser heads: S40/0.45: available working distance 40 mm, aperture 0.45 S80/0.30: available working distance 80 mm, aperture 0.30 | ● | ● | |
| Contrasting | Precentered insert with four positions Insert for IMC illumination | (Bright? eld, 5x–63 x Phase Contrast)(Bright? eld, 10x, 20x, 32x, 60x IMC) | ● | ● |
| Contrast methods | Bright? eld, Phase Contrast, Integrated Modulation Contrast | ● | ● | |
| Fluorescence | Fluorescence slider with three positions for ? lter cubes Manual light stop | ● | ○ | |
| Fluorescence illumination | Fluorescence LED Leica SFL100, 50W Hg, 100W Hg, Leica EL6000 ?beroptic coupling | ● | ○ | |
| Stages | Fixed stage, ?xed heating stage, 3-plate stage, attachable mechanical stage for both ?xed stages | ● | ● | |
| Documentation | Camera port for all Leica digital cameras and common camera models | ● | ● | |
| Tubes | Binocular tube 45°, interpupillary distance 55–75 mm, ? eld of view 20 mm | ● | ● | |
| Tubes | Trinocular phototube 45°, interpupillary distance 55–75 mm, ? eld of view 20 mm, | ● | ● | |
| with camera port positioned 88 mm to the side, selectable 99% photo or 99% visual | ● | ● | ||
| Additional options in the Leica DM product line: Standard binocular tube 30°, Ergo binocular tube 15° Ergo Vario binocular tube 7.5–15°, Ergo Vario binocular tube 0–55° Ergo Vario binocular tube 5°–32° and eyepiece extension 0–30 mm Standard trinocular phototube 30°, Ergo Vario trinocular phototube 0°–35°
| ● | ● | ||
