技術(shù)原理：
xCELLigence RTCA DP 多功能實(shí)時(shí)無標(biāo)記細(xì)胞分析儀基于艾森生物（ACEA Biosciences）核心技術(shù)——實(shí)時(shí)無標(biāo)記動(dòng)態(tài)細(xì)胞分析技術(shù)，實(shí)時(shí)、動(dòng)態(tài)、定量跟蹤細(xì)胞的遷移及浸潤的動(dòng)力學(xué)檢測。該技術(shù)采用特殊工藝，將微電子細(xì)胞傳感器芯片整合到細(xì)胞浸潤遷移板（CIM Plate) 的微孔膜下層，從而便利并精確地檢測細(xì)胞的遷移及浸潤。
應(yīng)用領(lǐng)域：
1、細(xì)胞浸潤及遷移 2、受體介導(dǎo)的信號(hào)通路
3、細(xì)胞與細(xì)胞相互作用 4、細(xì)胞增殖及分化
5、細(xì)胞粘附及伸展 6、病毒介導(dǎo)的細(xì)胞病變
7、化合物及細(xì)胞因子介導(dǎo)的細(xì)胞毒作用 8、NK細(xì)胞介導(dǎo)的細(xì)胞殺傷作用及ADCC
應(yīng)用實(shí)例：
實(shí)時(shí)監(jiān)測Hela細(xì)胞浸潤 實(shí)時(shí)監(jiān)測HUVEC細(xì)胞遷移
圖A: HUVEC細(xì)胞呈現(xiàn)VEGF濃度依賴的細(xì)胞遷移
圖B: xCELLigence DP 系統(tǒng)軟件自動(dòng)計(jì)算的兩個(gè)不同時(shí)間點(diǎn)的EC50值
xCELLigence RTCA DP 實(shí)時(shí)無標(biāo)記細(xì)胞功能分析儀的技術(shù)優(yōu)勢：
1、無需標(biāo)記，對(duì)細(xì)胞無損傷，在生理狀態(tài)下進(jìn)行檢測，結(jié)果準(zhǔn)確度高。
2、自動(dòng)、連續(xù)監(jiān)測，同時(shí)檢測短期（數(shù)分鐘）和長期（數(shù)周）細(xì)胞效應(yīng)，獲取全過程動(dòng)態(tài)信息。
3、交叉式電極設(shè)計(jì)，確保高精確性和高重復(fù)性，提供更大的動(dòng)態(tài)檢測范圍。
4、完整細(xì)胞效應(yīng)圖譜，提供大量、重要的動(dòng)態(tài)反應(yīng)信息，具有重要指導(dǎo)意義。
5、活細(xì)胞質(zhì)量控制，真正實(shí)現(xiàn)自身對(duì)照參考。
xCELLigence RTCA DP實(shí)時(shí)無標(biāo)記細(xì)胞功能分析儀的儀器特點(diǎn)：
4. Vosjan MJ., et al. Nanobodies targeting the hepatocyte growth factor: potential new drugs for molecular cancer therapy.Mol Cancer Ther. 2012 Apr;11:1017-25.
6. Kapp GT., et al. Control of protein signaling using a computationally designed GTPase/GEF orthogonal pair. Proc Natl Acad Sci U S A. 2012 Apr 3;109:5277-82.
8. Rammah M., et al. In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells. Toxicol Lett. 2012 Jun 20;211:220-31.
xCELLigence RTCA DP實(shí)時(shí)無標(biāo)記細(xì)胞功能分析儀的儀器特點(diǎn)：
緊湊型設(shè)計(jì)，體積小巧，節(jié)省空間。
高靈活性3個(gè)獨(dú)立E-Plate 16模塊，可同時(shí)滿足不同研究者的應(yīng)用需求。
讀取整個(gè)E-Plate 16檢測最多只需要4秒，捕獲細(xì)胞響應(yīng)信號(hào)的準(zhǔn)確度高。
支持從細(xì)胞遷移、浸潤到細(xì)胞毒作用等多種檢測應(yīng)用，功能更靈活。
1.2版本軟件，添加網(wǎng)絡(luò)功能，可對(duì)IC50、EC50等數(shù)據(jù)進(jìn)行自動(dòng)計(jì)算。
設(shè)備安裝簡便，即插即用，便以維護(hù)。
xCELLigence RTCA DP實(shí)時(shí)無標(biāo)記細(xì)胞功能分析儀及耗材：
RTCA DP 檢測分析儀 E-Plate 16檢測板 CIM-Plate 16 檢測板 E-Plate Insert
參考文獻(xiàn)：
RTCA DP 檢測分析儀 E-Plate 16檢測板 CIM-Plate 16 檢測板 E-Plate Insert
參考文獻(xiàn)：
細(xì)胞浸潤與遷移（ Cell Invasion and Migration ）
1. Gleize V., et al. The renal v-ATPase a4 subunit is expressed in specific subtypes of human gliomas. Glia. 2012 May; 60:1004-12.
2. Hellevik T., et al. Cancer-associated fibroblasts from human NSCLC survive ablative doses of radiation but their invasive capacity is reduced. Radiat Oncol. 2012 Apr 13; 7:59. 細(xì)胞增殖（ Cell Proliferation ）
3. Kaschula CH., et al. Structure-activity studies on the anti-proliferation activity of ajoene analogues in WHCO1 oesophageal cancer cells. Eur J Med Chem. 2012 Apr;50:236-54.4. Vosjan MJ., et al. Nanobodies targeting the hepatocyte growth factor: potential new drugs for molecular cancer therapy.Mol Cancer Ther. 2012 Apr;11:1017-25.
受體激活（ Receptor Activation ）
5. Yoshioka H., et al.Possible aryl hydrocarbon receptor-independent pathway of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced antiproliferative response in human breast cancer cells. Toxicol Lett. 2012 Jun 20;211:257-265.6. Kapp GT., et al. Control of protein signaling using a computationally designed GTPase/GEF orthogonal pair. Proc Natl Acad Sci U S A. 2012 Apr 3;109:5277-82.
細(xì)胞毒性與細(xì)胞死亡（ Cytotoxicity and Cell Death ）
7. Gerets HH., et al. Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins. Cell Biol Toxicol. 2012 Apr;28:69-87.8. Rammah M., et al. In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells. Toxicol Lett. 2012 Jun 20;211:220-31.
免疫學(xué)（ Immunology ）
9. Chou J., et al. Epigenetic modulation to enable antigen-specific T-cell therapy of colorectal cancer. J Immunother. 2012 Feb-Mar;35:131-41. 10. Miller TW., et al. Hydrogen sulfide is an endogenous potentiator of T cell activation. J Biol Chem. 2012 Feb 3;287:4211-21
