詳細介紹
Column: | Sepax AA, 4.6×250 mm |
phase: | A: ACN : 0.1 M NaAc = 7 : 93 (v/v), pH=6.5; |
Time (min): | 0 15 18 25 30 30.01 45 |
Flow rate: | 1.0 mL/min |
Temperature: | 36 oC |
Detection: | UV 254nm |
Injection Volume: | 5 μL |
Sample: | 18 Amino acids |
1.Asp | 2.Glu | 3.Ser | 4.Gly | 5.His | 6.Arg |
7.Thr | 8.Ala | 9.Pro | 10.Tyr | 11.Val | 12.Met |
13.Cys | 14.Ile | 15.Leu | 16.Phe | 17.Trp | 18.Lys |