人10kDa熱休克蛋白1檢測試劑盒定量檢測,具有靈敏性高,特異性強,重復(fù)性好,性價比高,線性好;操作簡單,精確度高;售前售后完善,隨時來實驗室現(xiàn)場觀看,常見ELISA試劑盒系列:人,大鼠,小鼠:TNF-α、IL-1β、IL-7、IL-32、IL-21、IL-27、IL-23、IFN-γ、TGF-α、TGF-β1等。
英文簡稱:Human Heat Shock 10kDa Protein 1 (HSP10) ELISA Kit
人10kDa熱休克蛋白1檢測試劑盒注意事項以下四點:
1,封閉:以下包以后是無關(guān)的蛋白質(zhì)溶液濃度高,涂層技術(shù)。隨函附上使許多不相關(guān)的蛋白質(zhì)充填這些空隙,和攪擾物質(zhì)的免疫排擠和吸附進程。ELISA試劑盒常用的密封:0。05% - 0。5%牛血清白蛋白;10%或1%明膠牛血清;脫脂奶粉,報價相對低廉,可用于高濃度(5%~10%);和一些稀有的運用各種動物血清(首要是為了消除類似的蛋白和酪蛋白等攪擾)。但究竟挑選啥,依據(jù)試驗的詳細(xì)實習(xí)。
2,洗板:能夠說,在中操作,清洗是在首要的關(guān)鍵技術(shù)。因為聚苯乙烯等塑料對蛋白質(zhì)的吸附是普遍存在的,為了完成自在酶與客觀相的符號的別離,在板料孔中殘留游離和吸附的非特異性攪擾物質(zhì)的去掉,洗刷,并應(yīng)將攪擾物質(zhì)洗刷下來的非特異性吸附。所以在洗衣板會有必定的誤差,非常人的要素(當(dāng)然也有洗衣機除條件),是不完整的或弦清孔,體系的影響是如此靈敏,但不小。
3,加抗體(抗標(biāo)本和兩個):留意的槍頭,槍頭。樣品用稀釋稀釋,也能夠用稀釋的閉解。假如要增加兩個電阻,但也要留意兩個反作業(yè)濃度的廢物,太高,太低的光的色彩。
4,色彩:色彩體系有許多,咱們開端做,挑選恰當(dāng)?shù)纳鼠w系。ELISA試劑盒留意色彩體系酶底物物加硫柳泵節(jié)省,組合物加*。7,每次都盡可能的陰和陽空三操控,如剖析疑問的呈現(xiàn)。
YB-PKR1-Mu 小鼠前動力蛋白受體1(PKR1) ELISA Kit Mouse Prokineticin Receptor 1 (PKR1) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.04ng/mL sandwich 96T/48T 905/633.5 12 months
YB-PKR1-Ra 大鼠前動力蛋白受體1(PKR1) ELISA Kit Rat Prokineticin Receptor 1 (PKR1) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.039ng/mL sandwich 96T/48T 950/665 12 months
YB-PKR2-Hu 人前動力蛋白受體2(PKR2) ELISA Kit Human Prokineticin Receptor 2 (PKR2) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.107ng/mL sandwich 96T/48T 882.5/617.7 12 months
YB-PK-Ra 大鼠丙酮酸激酶(PK) ELISA Kit Rat Pyruvate Kinase, Liver And RBC (PK) ELISA Kit Rattus norvegicus Rat 0.781-50ng/mL 0.35ng/mL sandwich 96T/48T 950/665 12 months
YB-PKR-Hu 人蛋白激酶R(PKR) ELISA Kit Human Protein Kinase R (PKR) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.055ng/mL sandwich 96T/48T 882.5/617.7 12 months
YB-PLA1-Hu 人磷脂酶A1(PLA1) ELISA Kit Human Phospholipase A1 (PLA1) ELISA Kit Homo sapiens human 78.125-5000pg/mL 3.4pg/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PLA2G10-Hu 人Ⅹ組磷脂酶A2(PLA2G10) ELISA Kit Human Phospholipase A2, Group X (PLA2G10) ELISA Kit Homo sapiens human 78.125-5000pg/mL 3.7pg/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PLA2G12B-Hu 人ⅫB組磷脂酶A2(PLA2G12B) ELISA Kit Human Phospholipase A2, Group XIIB (PLA2G12B) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.058ng/mL sandwich 96T/48T 1057.5/740.3 12 months
YB-PLA2G2A-Hu 人ⅡA組磷脂酶A2(PLA2G2A) ELISA Kit Human Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit Homo sapiens human 6.25-400pg/mL 2.55pg/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PLA2G2A-Mu 小鼠ⅡA組磷脂酶A2(PLA2G2A) ELISA Kit Mouse Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 5.7pg/mL sandwich 96T/48T 995/696.5 12 months
YB-PLA2G2A-Ra 大鼠ⅡA組磷脂酶A2(PLA2G2A) ELISA Kit Rat Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit Rattus norvegicus Rat 0.312-20ng/mL 0.116ng/mL sandwich 96T/48T 1045.1/731.6 12 months
YB-PLA2G2D-Hu 人ⅡD組磷脂酶A2(PLA2G2D) ELISA Kit Human Phospholipase A2, Group IID (PLA2G2D) ELISA Kit Homo sapiens human 0.937-60ng/mL 0.32ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PLA2G2D-Mu 小鼠ⅡD組磷脂酶A2(PLA2G2D) ELISA Kit Mouse Phospholipase A2, Group IID (PLA2G2D) ELISA Kit Mus musculus mouse 31.25-2000pg/mL 12.4pg/mL sandwich 96T/48T 950/665 12 months
YB-PLA2G3-Mu 小鼠Ⅲ組磷脂酶A2(PLA2G3) ELISA Kit Mouse Phospholipase A2, Group III (PLA2G3) ELISA Kit Mus musculus mouse 0.781-50ng/mL 0.27ng/mL sandwich 96T/48T 995/696.5 12 months
YB-PLA2G4D-Hu 人ⅣD組磷脂酶A2(PLA2G4D) ELISA Kit Human Phospholipase A2, Group IVD (PLA2G4D) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.064ng/mL sandwich 96T/48T 1057.5/740.3 12 months
YB-PLA2G5-Hu 人Ⅴ組磷脂酶A2(PLA2G5) ELISA Kit