人11-β-羥基類固醇脫氫酶3檢測試劑盒定量檢測,具有靈敏性高,特異性強,重復(fù)性好,性價比高,線性好;操作簡單,精確度高;售前售后完善,隨時來實驗室現(xiàn)場觀看,常見ELISA試劑盒系列:人,大鼠,小鼠:TNF-α、IL-1β、IL-7、IL-32、IL-21、IL-27、IL-23、IFN-γ、TGF-α、TGF-β1等。
英文簡稱:Human 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) ELISA Kit
人11-β-羥基類固醇脫氫酶3檢測試劑盒注意事項以下四點:
1,封閉:以下包以后是無關(guān)的蛋白質(zhì)溶液濃度高,涂層技術(shù)。隨函附上使許多不相關(guān)的蛋白質(zhì)充填這些空隙,和攪擾物質(zhì)的免疫排擠和吸附進程。ELISA試劑盒常用的密封:0。05% - 0。5%牛血清白蛋白;10%或1%明膠牛血清;脫脂奶粉,報價相對低廉,人11-β-羥基類固醇脫氫酶3檢測試劑盒可用于高濃度(5%~10%);和一些稀有的運用各種動物血清(首要是為了消除類似的蛋白和酪蛋白等攪擾)。但究竟挑選啥,依據(jù)試驗的詳細實習(xí)。
2,洗板:能夠說,在中操作,清洗是在首要的關(guān)鍵技術(shù)。因為聚苯乙烯等塑料對蛋白質(zhì)的吸附是普遍存在的,為了完成自在酶與客觀相的符號的別離,在板料孔中殘留游離和吸附的非特異性攪擾物質(zhì)的去掉,洗刷,并應(yīng)將攪擾物質(zhì)洗刷下來的非特異性吸附。所以在洗衣板會有必定的誤差,非常人的要素(當(dāng)然也有洗衣機除條件),是不完整的或弦清孔,體系的影響是如此靈敏,但不小。
3,人11-β-羥基類固醇脫氫酶3檢測試劑盒加抗體(抗標(biāo)本和兩個):留意的槍頭,槍頭。樣品用稀釋稀釋,也能夠用稀釋的閉解。假如要增加兩個電阻,但也要留意兩個反作業(yè)濃度的廢物,太高,太低的光的色彩。
4,色彩:色彩體系有許多,咱們開端做,挑選恰當(dāng)?shù)纳鼠w系。ELISA試劑盒留意色彩體系酶底物物加硫柳泵節(jié)省,組合物加*。7,每次都盡可能的陰和陽空三操控,如剖析疑問的呈現(xiàn)。YB-PIK3Cd-Hu 人磷酸肌醇-3-激酶催化亞基δ肽(PIK3Cδ) ELISA Kit Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.086ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PIM1-Hu 人Pim-1原癌基因(PIM1) ELISA Kit Human Pim-1 Oncogene (PIM1) ELISA Kit Homo sapiens human 0.781-50ng/mL 0.25ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PIM2-Hu 人Pim-2原癌基因(PIM2) ELISA Kit Human Pim-2 Oncogene (PIM2) ELISA Kit Homo sapiens human 0.625-40ng/mL 0.227ng/mL sandwich 96T/48T 1057.5/740.3 12 months
YB-PIM3-Hu 人Pim-3原癌基因(PIM3) ELISA Kit Human Pim-3 Oncogene (PIM3) ELISA Kit Homo sapiens human 0.625-40ng/mL 0.228ng/mL sandwich 96T/48T 1057.5/740.3 12 months
YB-PINP-Hu 人Ⅰ型前膠原氨基端原肽(PⅠNP) ELISA Kit Human Procollagen I N-Terminal Propeptide (PINP) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.4pg/mL sandwich 96T/48T 882.5/617.7 12 months
YB-PINP-Mu 小鼠Ⅰ型前膠原氨基端原肽(PⅠNP) ELISA Kit Mouse Procollagen I N-Terminal Propeptide (PINP) ELISA Kit Mus musculus mouse 93.75-6000pg/mL 35.57pg/mL sandwich 96T/48T 905/633.5 12 months
YB-PINP-Ra 大鼠Ⅰ型前膠原氨基端原肽(PⅠNP) ELISA Kit Rat Procollagen I N-Terminal Propeptide (PINP) ELISA Kit Rattus norvegicus Rat 0.625-40ng/mL 0.215ng/mL sandwich 96T/48T 950/665 12 months
YB-PIP-Hu 人催乳素誘導(dǎo)蛋白(PIP) ELISA Kit Human Prolactin Induced Protein (PIP) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.061ng/mL sandwich 96T/48T 1057.5/740.3 12 months
YB-PIP-Ra 大鼠催乳素誘導(dǎo)蛋白(PIP) ELISA Kit Rat Prolactin Induced Protein (PIP) ELISA Kit Rattus norvegicus Rat 46.875-3000pg/mL 16.23pg/mL sandwich 96T/48T 1140/798 12 months
YB-PK2-b 牛前動力蛋白2(PK2) ELISA Kit Bovine Prokineticin 2 (PK2) ELISA Kit Bos taurus; Bovine Cattle 1.25-80ng/mL 0.45ng/mL sandwich 96T/48T 1040/728 12 months
YB-PK2-Hu 人前動力蛋白2(PK2) ELISA Kit Human Prokineticin 2 (PK2) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.084ng/mL competitive inhibition 96T/48T 882.5/617.7 12 months
YB-PK2-Mu 小鼠前動力蛋白2(PK2) ELISA Kit Mouse Prokineticin 2 (PK2) ELISA Kit Mus musculus mouse 0.312-20ng/mL 0.088ng/mL competitive inhibition 96T/48T 905/633.5 12 months
YB-PK2-Ra 大鼠前動力蛋白2(PK2) ELISA Kit Rat Prokineticin 2 (PK2) ELISA Kit Rattus norvegicus Rat 0.312-20ng/mL 0.078ng/mL sandwich 96T/48T 950/665 12 months
YB-PKBa-Hu 人蛋白激酶Bα(PKBα) ELISA Kit Human Protein Kinase B Alpha (PKBa) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.053ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PKBa-Mu 小鼠蛋白激酶Bα(PKBα) ELISA Kit