Anti-HMGA1(高遷移率族蛋白A1抗體))
專業(yè)提供Anti-HMGA1(高遷移率族蛋白A1抗體))廣泛應(yīng)用于免疫組化,ELISA,Western Blot IHC-F(免疫組化-冰凍切片),IHC-P (免疫組化-石蠟切片)IP(免疫沉淀), Flow-Cyt等方面。
抗體交叉反應(yīng)的原因:
指抗體除與其相應(yīng)的抗原發(fā)生特異性反應(yīng)外還與其它抗原發(fā)生反應(yīng)。產(chǎn)生的原因有以下幾個方面:
1. 抗原特異性指用于免疫動物的抗原性物質(zhì)中含有多種抗原分子,它引起動物產(chǎn)生針對多種抗原分子特異性的相應(yīng)抗體。任何其它物質(zhì)只要含有一種或多種與上述物質(zhì)相同的抗原分子,必將與上述多特異性的抗血清發(fā)生交叉反應(yīng)。
2. 共同決定簇即兩種抗原分子中都含有相同的抗原決定簇。
3. 決定簇相似,兩種不同的抗原決定簇,如果結(jié)構(gòu)大致相同,由于空間構(gòu)象關(guān)系,某一決定簇的相應(yīng)抗體可以與大致相同的決定簇發(fā)生交叉反應(yīng)。當(dāng)然抗原一抗體之間構(gòu)象相似時的結(jié)合力小于吻合時的結(jié)合力。
產(chǎn)品詳細信息:
產(chǎn)品編號 Rs-1030R
英文名稱 Anti-HMGA1
中文名稱 高遷移率族蛋白A1抗體
抗體介紹:HMGA1 (HMGI/Y; high mobility group AT-hook 1) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex.
別 名 high mobility group AT-hook 1; HMG-I/HMG-Y; HMG-R; HMGA 1a; HMGA 1b; HMGA1; HMGI; HMGI/Y; HMGIY; HMGY; MGC12816; MGC4242; MGC4854.
產(chǎn)品類型 一抗
抗體來源 Rabbit
克隆號 Polyclonal
交叉反應(yīng) hu, mo, rat, bov, chi
蛋白分子量 Predicted Molecular Weight: 10kDa
性狀 Lyophilized or Liquid
濃度 1mg/1ml
免疫原 KLH conjugated synthetic peptide derived from human HMGA1 C-terminus.
亞型 IgG
產(chǎn)品應(yīng)用 WB=1:100-500 Elisa=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1/20-1/100
(石蠟切片需做抗原修復(fù))
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
公司提供的)已被國內(nèi)外廣大科研工作者使用,廣泛用于分子生物學(xué)、免疫學(xué)等試驗中。
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