上海研盟生物科技有限公司PAX5抗體-配對盒基因5抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細胞術(shù)等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/p>
簡單介紹:
中文名稱:配對盒基因5抗體
英文名稱:Anti-PAX5
產(chǎn)品編號:BYK-1861R
產(chǎn)品別名:B cell lineage specific activator; B cell lineage specific activator protein; B cell specific activator protein; B cell specific transcription factor; B-cell-specific transcription factor; BSAP; EBB-1; KLP; Paired box 5; Paired box gene 5 (B cell lineage specific activator protein); Paired box gene 5 (B cell lineage specific activator); Paired box gene 5; Paired box homeotic gene 5; Paired box protein Pax 5; Paired box protein Pax-5; Paired domain gene 5; PAX 5; PAX5; PAX5_HUMAN; Transcription factor PAX 5.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實驗
產(chǎn)品價格:請報價
說明書:請?zhí)砑涌头苯铀魅?/p>
抗體來源:該指標有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PAX5抗體-配對盒基因5抗體產(chǎn)品介紹:The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
多肽的溶解與保存
一、凍干多肽的溶解
1、多肽溶解的主要問題是二級結(jié)構(gòu)的形成。雖然疏水肽鏈二級結(jié)構(gòu)的形成更明顯,但除zui短的肽鏈外,此現(xiàn)象產(chǎn)生在幾乎所有肽鏈,與極性無關(guān)。所以,溶解多肽的*個原則是使用無菌蒸餾水或去離子水,當然無氧水。多肽溶液可能遇到細菌降解,為防止此情況的發(fā)生,應(yīng)溶解在無菌的蒸餾水中或用0.45或0.2孔徑的濾膜過濾除菌。含有Cys、Met、Trp的多肽很容易氧化,應(yīng)溶于無氧的水中,無氧水可通過注入惰性氣體(氮、氦、氬)減壓除氣得到;
2、若多肽不溶于純水,超聲處理有助于打碎顆粒并增加溶解度。注意:超聲處理會引起溶液發(fā)熱或多肽降解;
3、若多肽含有多個堿性氨基酸,使用(1-10%)的乙酸水溶液:對于疏水性強的多肽,應(yīng)使用50%的乙酸;
4、若多肽中含有大量酸性氨基酸,可用氨溶液(1-10%)或乙酸乙基*或重碳酸鹽等揮發(fā)性的堿性緩沖液溶解。pH值在層析前必須調(diào)整;
5、異丙醇和乙腈能溶解中等大小的多肽。若肽要上柱,有機溶劑的量必須很少否則將嚴重影響停留時間;
6、若多肽因含有Val、Leu、Met、Phe、Tyr、Ala等芳香鏈而高度疏水、或為中性肽時,DMF或DMSO等膜變性劑的使用,有利于多肽的溶解:
a.高濃度模變性劑通過破壞多肽的二級結(jié)構(gòu)而助溶;
b.膜變性劑適于多肽分析液的制備,但可能會對其生物活性的研究工作造成干擾;
c.DMF是變性劑(zui高濃度可達30%),滴加至多肽溶解;
d.反相層析法時,DMF將與洗脫液前鋒一起流出,根據(jù)入量的多少,峰值可能很高。大多數(shù)肽能在大量DMF流出后幾分鐘內(nèi)流出,若肽鏈很小,洗脫太早,多肽的量將很低。
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