中文名稱:細(xì)胞分裂周期蛋白25抗體
英文名稱:Anti-Cdc25A
產(chǎn)品編號(hào):BYK-2758R
別名:Cdc 25a; CDC25A2; Cell division cycle 25 homolog A (S. pombe); Cell division cycle 25A; Cell division cycle 25A isoform a; Cell division cycle 25A isoform b; D9Ertd393e; Dual specificity phosphatase CDC25A; EC 3.1.3.48; M phase inducer phosphatase 1; MGC115549; CDC25A; CDC25A2 CAG isoform; M-phase inducer phosphatase 1; MPIP1_HUMAN.
產(chǎn)品介紹:CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
Cdc25A抗體-細(xì)胞分裂周期蛋白25抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融,保質(zhì)期一年。
Cdc25A抗體-細(xì)胞分裂周期蛋白25抗體對(duì)研究人員的多肽設(shè)計(jì)提供一些建議:
一、變難為易
1、減少序列長(zhǎng)度
肽的長(zhǎng)度增加導(dǎo)致粗產(chǎn)物純度降低,小于15個(gè)殘基的肽較容易得到.當(dāng)肽鏈增加到20個(gè)以上時(shí),正常產(chǎn)物的量就要考慮。在具體實(shí)踐中殘基低于20往往能得到更好的結(jié)果;
2、減少疏水殘基
疏水殘基占明顯優(yōu)勢(shì)的肽,尤其在距C端7―12個(gè)殘基的區(qū)域,會(huì)引起合成困難,一般認(rèn)為由于合成中形成β折疊片,而產(chǎn)生不*配對(duì)。當(dāng)然,可用幾個(gè)極性殘基置換,或加入Gly或Pro易打開肽結(jié)構(gòu)可能會(huì)得到幫助;
3、減少“難度”殘基
有多個(gè)Cys、Met、Arg、Try殘基一般難以合成,Ser通??勺鳛榉茄趸鎿Q。
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