上海研盟生物科技有限公司Anti-Methamphetamine抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。:
中文名稱:Methamphetamine抗體
英文名稱:Anti-Methamphetamine(4D2)抗體
產(chǎn)品編號(hào):BYK-2068M
別名:d-Desoxyephedrine hydrochloride; d-N; α-Dimethylphenethylamine hydrochloride; Methylamphetamine hydrochloride; METH.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
Anti-Methamphetamine抗體產(chǎn)品介紹:Methamphetamine (METH) is closely related chemically to amphetamine (AMPH). METH is a potent central nervous system stimulant with additional peripheral sympathomimetic effects. METH and AMPH have been used clinically in the treatment of obesity, minimal brain dysfunction, narcolepsy, depression and to counter fatigue. They are also subjected to widespread abuse. METH is an indirect agonists. It causes the release of newly synthesized norepinephrine and dopamine and it blocks the re uptake of these transmitters from the synapse. This can lead to an increase in the concentration of catecholamines in the synapse as well as an overall increase in catecholaminergic activity in the brain. The mechanism of METH induced neurotoxicity for all monoaminergic cell types may lie primarily with the dopaminergic system in the striatum. It may also lie with the interaction between METH induced release of dopamine and its ability to inhibit monoamine oxidase.
上海研盟生物科技有限公司實(shí)驗(yàn)注意事項(xiàng)
1、 把聚丙烯酰胺凝膠中的蛋白質(zhì)電泳轉(zhuǎn)移到硝酸纖維膜上;
a. 轉(zhuǎn)移緩沖液洗滌凝膠和硝酸纖維素膜,將硝酸纖維素膜鋪在凝膠上,用5ml移液管在凝膠上來回滾動(dòng)去除所有的氣泡
b. 在凝膠/濾膜外再包一張3mm濾紙(預(yù)先用轉(zhuǎn)移緩沖液浸濕),將凝膠夾在中間,保持濕潤和沒有氣泡
c. 將此濾紙/凝膠/薄膜濾紙按照廠家建議方法放入電泳裝置中,凝膠面向陰極
d. 將上述裝置放入緩沖液槽中,并灌滿轉(zhuǎn)移緩沖液以淹沒凝膠
e. 按照廠家所示接通電源開始電泳轉(zhuǎn)移
f. 轉(zhuǎn)移結(jié)束后,取出薄膜和凝膠,棄去凝膠
2、 將薄膜漂在氨基黑中快速染色,直至分子量標(biāo)準(zhǔn)顯現(xiàn)時(shí)取出,記錄下標(biāo)準(zhǔn)位置;
3、 用100ml水洗滌纖維素膜,必要時(shí)可用脫色緩沖液;
4、 膜置印跡緩沖液中于37℃保溫1小時(shí);
5、 室溫下,用PBS-Tween緩沖液洗滌薄膜
6、 用封口機(jī)將薄膜封入塑料袋中,盡可能不留空氣;
7、 袋的一角剪一緩沖液的小口,用透析袋夾緊;
8、 混合:NGS(100微升),印跡緩沖液中的抗體(2.5-5毫升),加在裝薄膜的袋中,于室溫下?lián)u動(dòng)2小時(shí)(或4℃過夜);
9、 用總體積300ml PBS-Tween緩沖液,分4次在一淺盤中洗滌薄膜,每次75ml;
10、將連接*的羊抗兔IgG(40微升溶于10毫升印跡緩沖液/100微升 NGS)加在袋內(nèi),于室溫下?lián)u動(dòng)1小時(shí);
11、按步驟9洗滌;
12、加入抗生素蛋白-HRP(40微升溶于10毫升印跡緩沖液/100微升 NGS),于室溫下?lián)u動(dòng);
13、Western Blot中轉(zhuǎn)移在膜上的蛋白處于變性狀態(tài),空間結(jié)構(gòu)改變,因此那些識(shí)別空間表位的抗體不能用于Western Blot檢測(cè)。這種情況可以將表達(dá)目的蛋白的細(xì)胞或細(xì)胞裂解液中的所有蛋白物素化,再用酶標(biāo)記親和素進(jìn)行Western Blot。實(shí)驗(yàn)中取膠和膜需帶手套。
相關(guān)優(yōu)質(zhì)產(chǎn)品:
LCAT(lecithin cholesterol acyl transferase) 卵磷*?;D(zhuǎn)移酶抗體
L-Citrulline 抗L-瓜氨酸抗體
Lck/p56-LCK(Lymphocyte cell-specific) 淋巴細(xì)胞特異性蛋白*激酶抗體
LDL-R (Low-density lipoprotein receptor precuBYKor) 低密度脂蛋白受體抗體
ox-LDL(Cu2SO4 oxidized low density lipoprotein) 抗氧化低密度脂蛋白抗體
LDH(Lactate DeYMdrogenase) 抗乳酸脫氫酶抗體
Lectin (Mannan-binding lectin; L-MBP) 抗凝集素抗體
LEF-1(lymphoid enhancing factor-1) 淋巴增強(qiáng)因子-1抗體
Leptin 瘦素抗體
Leptin 瘦素抗體
Leptin R(Leptin receptor) 瘦素受體抗體
Leptin receptor(long) 瘦素受體抗體(長)
Leptin receptor(long) 瘦素受體抗體(長)
Leptin receptor(L、M、S) 瘦素受體抗體(長、中、短型)
L-enk 抗*腦啡肽抗體
Lgr5/GPR49(G protein coupled receptor 49) Lgr5/GPR49抗體
LH (Human Luteinizing Hormone) mouse monoclonal antibody 鼠抗促黃體生成素抗體
LH(Luteinizing Hormone) 抗促黃體生成素抗體
LH(mouse Luteinizing Hormonee Monoclonal) 小鼠抗促黃體生成素單克隆抗體
LH(Luteinizing Hormone) 抗促黃體生成素抗體
LH/CG Receptor(Luteinizing Hormone/Choriogonadotropin Receptor) 促黃體生成素受體抗體