ELISA的方法檢測多肽與小鼠水通道蛋白1(AQP-1)測定試劑盒的結(jié)合情況。準備將合成多肽(其長度大約為8~10個氨基酸)包被于ELISA板,再加入小鼠水通道蛋白1(AQP-1)測定試劑盒,一抗、酶標二抗。請問在包被的過程中應(yīng)采取哪種包被緩沖液?固相載體是否要特殊處理?封閉液用BSA可以嗎?
1、多肽抗原的包被一般需先使其與無關(guān)蛋白質(zhì)如牛血清白蛋白質(zhì)(BSA)等偶聯(lián),借助于偶聯(lián)物與固相載體的吸附,間接地結(jié)合到固相載體表面。
2、小鼠水通道蛋白1(AQP-1)測定試劑盒包被用抗原或抗體的濃度,包被的溫度和時間,包被液的pH等應(yīng)根據(jù)試驗的特點和材料的性質(zhì)而選定??贵w和蛋白質(zhì)抗原一般采用pH9.6的碳酸鹽緩沖液作為稀釋液,也有用pH7.2的磷酸鹽緩沖液及pH7~8的Tris-HCL緩沖液作為稀釋液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置過夜,37℃中保溫2小時被認為具有同等的包被效果。
3、zui常用的封閉劑是0.05%-0.5%的牛血清白蛋白,小鼠水通道蛋白1(AQP-1)測定試劑盒也有用10%的小牛血清或1%明膠作為封閉劑的。脫脂奶粉也是一種良好的封閉劑,其zui大的特點是價廉,可以高濃度使用(5%)。
顯色終止后出現(xiàn)黑色絮狀沉淀可能為包被板上的蛋白,酶標記物,檢測物,封閉液中蛋白變性以及顯色反應(yīng)TMB過量,不溶,沉淀出來。
小鼠水通道蛋白1(AQP-1)測定試劑盒其它優(yōu)勢產(chǎn)品如下:
klD822Mu 小鼠血小板反應(yīng)蛋白2(THBS2)ELISA試劑盒 ELISA Kit for Thrombospondin 2 (THBS2) Assay range:30 ng/L -1473 ng/L
klH819Mu 小鼠白介素1受體輔助蛋白(IL1RAP)ELISA試劑盒 ELISA Kit for Interleukin 1 Receptor Accessory Protein (IL1RAP) Assay range:30 ng/L -1474 ng/L
klC246Mu 小鼠磷酸肌醇依賴性蛋白激酶1(PDPK1)ELISA試劑盒 ELISA Kit for Phosphoinositide Dependent Protein Kinase 1 (PDPK1) Assay range:30 ng/L -1475 ng/L
klC150Mu 小鼠Ⅵ型膠原α1(COL6α1)ELISA試劑盒 ELISA Kit for Collagen Type VI Alpha 1 (COL6a1) Assay range:30 ng/L -1476 ng/L
klD761Mu 小鼠*合成酶(GS)ELISA試劑盒 ELISA Kit for Glutamine synthetase (GS) Assay range:30 ng/L -1477 ng/L
klB845Mu 小鼠CD83分子(CD83)ELISA試劑盒 ELISA Kit for Cluster Of Differentiation 83 (CD83) Assay range:30 ng/L -1478 ng/L
klB448Mu 小鼠信號素7A(SEMA7A)ELISA試劑盒 ELISA Kit for Semaphorin 7A (SEMA7A) Assay range:30 ng/L -1479 ng/L
klH574Mu 小鼠N-乙酰半乳糖胺酶α(NAGα)ELISA試劑盒 ELISA Kit for N-Acetylgalactosaminidase Alpha (NAGa) Assay range:30 ng/L -1480 ng/L
klB067Mu 小鼠YY肽(PYY)ELISA試劑盒 ELISA Kit for Peptide YY (PYY) Assay range:30 ng/L -1481 ng/L
klB127Mu 小鼠脫氧核糖核酸酶Ⅰ(DNASE1)ELISA試劑盒 ELISA Kit for Deoxyribonuclease I (DNASE1) Assay range:30 ng/L -1482 ng/L
klB929Mu 小鼠*乙酰轉(zhuǎn)移酶(ChAT)ELISA試劑盒 ELISA Kit for Choline Acetyltransferase (ChAT) Assay range:30 ng/L -1483 ng/L
klG918Mu 小鼠尿調(diào)蛋白(UMOD)ELISA試劑盒 ELISA Kit for Uromodulin (UMOD) Assay range:30 ng/L -1484 ng/L
klE112Mu 小鼠肝配蛋白B2(EFNB2)ELISA試劑盒 ELISA Kit for Ephrin B2 (EFNB2) Assay range:30 ng/L -1485 ng/L
klB166Mu 小鼠VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)ELISA試劑盒 ELISA Kit for VGF Nerve Growth Factor Inducible (VGF) Assay range:30 ng/L -1486 ng/L
klB267Mu 小鼠激肽原1(KNG1)ELISA試劑盒 ELISA Kit for Kininogen 1 (KNG1) Assay range:30 ng/L -1487 ng/L
klB607Mu 小鼠胸腎表達趨化因子(BRAK)ELISA試劑盒 ELISA Kit for Breast And Kidney Expressed Chemokine (BRAK) Assay range:30 ng/L -1488 ng/L
klJ026Mu 小鼠*酶(GLS)ELISA試劑盒 ELISA Kit for Glutaminase (GLS) Assay range:30 ng/L -1489 ng/L
klJ025Mu 小鼠*酶2(GLS2)ELISA試劑盒 ELISA Kit for Glutaminase 2 (GLS2) Assay range:30 ng/L -1490 ng/L
klD232Mu 小鼠心肌肌鈣蛋白T(TNNT2)ELISA試劑盒 ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) Assay range:30 ng/L -1491 ng/L
klB263Mu 小鼠*加壓素受體1B(AVPR1B)ELISA試劑盒 ELISA Kit for Arginine Vasopressin Receptor 1B (AVPR1B) Assay range:30 ng/L -1492 ng/L
klL643Mu 小鼠EGF樣域蛋白7(EGFL7)ELISA試劑盒 ELISA Kit for EGF Like Domain Protein, Multiple 7 (EGFL7) Assay range:30 ng/L -1493 ng/L
klG962Mu 小鼠翻譯調(diào)節(jié)腫瘤蛋白1(TPT1)ELISA試劑盒 ELISA Kit for Tumor Protein, Translationally Controlled 1 (TPT1) Assay range:30 ng/L -1494 ng/L
klB890Mu 小鼠載脂蛋白C3(APOC3)ELISA試劑盒 ELISA Kit for Apolipoprotein C3 (APOC3) Assay range:30 ng/L -1495 ng/L
klA243Mu 小鼠對氧磷酶1(PON1)ELISA試劑盒 ELISA Kit for Paraoxonase 1 (PON1) Assay range:30 ng/L -1496 ng/L
klC913Mu 小鼠成纖維細胞生長因子11(FGF11)ELISA試劑盒 ELISA Kit for Fibroblast Growth Factor 11 (FGF11) Assay range:30 ng/L -1497 ng/L
klC914Mu 小鼠成纖維細胞生長因子12(FGF12)ELISA試劑盒 ELISA Kit for Fibroblast Growth Factor 12 (FGF12) Assay range:30 ng/L -1498 ng/L
klC395Mu 小鼠羧肽酶A3(CPA3)ELISA試劑盒 ELISA Kit for Carboxypeptidase A3, Mast Cell (CPA3) Assay range:30 ng/L -1499 ng/L