ELISA的方法檢測多肽與小鼠組織因子途徑抑制物(TFPI)測定試劑盒的結(jié)合情況。準備將合成多肽(其長度大約為8~10個氨基酸)包被于ELISA板,再加入小鼠組織因子途徑抑制物(TFPI)測定試劑盒,一抗、酶標二抗。請問在包被的過程中應(yīng)采取哪種包被緩沖液?固相載體是否要特殊處理?封閉液用BSA可以嗎?
1、多肽抗原的包被一般需先使其與無關(guān)蛋白質(zhì)如牛血清白蛋白質(zhì)(BSA)等偶聯(lián),借助于偶聯(lián)物與固相載體的吸附,間接地結(jié)合到固相載體表面。
2、小鼠組織因子途徑抑制物(TFPI)測定試劑盒包被用抗原或抗體的濃度,包被的溫度和時間,包被液的pH等應(yīng)根據(jù)試驗的特點和材料的性質(zhì)而選定。抗體和蛋白質(zhì)抗原一般采用pH9.6的碳酸鹽緩沖液作為稀釋液,也有用pH7.2的磷酸鹽緩沖液及pH7~8的Tris-HCL緩沖液作為稀釋液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置過夜,37℃中保溫2小時被認為具有同等的包被效果。
3、zui常用的封閉劑是0.05%-0.5%的牛血清白蛋白,小鼠組織因子途徑抑制物(TFPI)測定試劑盒也有用10%的小牛血清或1%明膠作為封閉劑的。脫脂奶粉也是一種良好的封閉劑,其zui大的特點是價廉,可以高濃度使用(5%)。
顯色終止后出現(xiàn)黑色絮狀沉淀可能為包被板上的蛋白,酶標記物,檢測物,封閉液中蛋白變性以及顯色反應(yīng)TMB過量,不溶,沉淀出來。
小鼠組織因子途徑抑制物(TFPI)測定試劑盒其它優(yōu)勢產(chǎn)品如下:
klC242Mu 小鼠核仁素(NCL)ELISA試劑盒 ELISA Kit for Nucleolin (NCL) Assay range:30 ng/L -1355 ng/L
klC363Mu 小鼠染色框同源物3(CBX3)ELISA試劑盒 ELISA Kit for Chromobox Homolog 3 (CBX3) Assay range:30 ng/L -1356 ng/L
klC664Mu 小鼠核仁磷酸蛋白(NPM)ELISA試劑盒 ELISA Kit for Nucleophosmin (NPM) Assay range:30 ng/L -1357 ng/L
klC684Mu 小鼠2',5'-寡腺苷酸合成酶1(OAS1)ELISA試劑盒 ELISA Kit for 2',5'-Oligoadenylate Synthetase 1 (OAS1) Assay range:30 ng/L -1358 ng/L
klA692Mu 小鼠神經(jīng)纖維網(wǎng)蛋白1(NRP1)ELISA試劑盒 ELISA Kit for Neuropilin 1 (NRP1) Assay range:30 ng/L -1359 ng/L
klB254Mu 小鼠血管抑素(ANG)ELISA試劑盒 ELISA Kit for Angiostatin (ANG) Assay range:30 ng/L -1360 ng/L
klE624Mu 小鼠周期素依賴性激酶抑制因子1A(CDKN1A)ELISA試劑盒 ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Assay range:30 ng/L -1361 ng/L
klA636Mu 小鼠成纖維細胞生長因子7(FGF7)ELISA試劑盒 ELISA Kit for Fibroblast Growth Factor 7 (FGF7) Assay range:30 ng/L -1362 ng/L
klB404Mu 小鼠Thy1細胞表面抗原(Thy1)ELISA試劑盒 ELISA Kit for Thy1 Cell Surface Antigen (Thy1) Assay range:30 ng/L -1363 ng/L
klA985Mu 小鼠P-選擇素糖蛋白配體1(PSGL1)ELISA試劑盒 ELISA Kit for P-Selectin Glycoprotein Ligand 1 (PSGL1) Assay range:30 ng/L -1364 ng/L
klJ130Mu 小鼠V-Ets骨髓成紅細胞增多癥病毒E26癌基因同源物1(ETS1)ELISA試劑盒 ELISA Kit for V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) Assay range:30 ng/L -1365 ng/L
klF878Mu 小鼠分泌型卷曲相關(guān)蛋白4(SFRP4)ELISA試劑盒 ELISA Kit for Secreted Frizzled Related Protein 4 (SFRP4) Assay range:30 ng/L -1366 ng/L
klA717Mu 小鼠次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)ELISA試劑盒 ELISA Kit for Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) Assay range:30 ng/L -1367 ng/L
klG435Mu 小鼠橋粒芯膠粘蛋白1(DSC1)ELISA試劑盒 ELISA Kit for Desmocollin 1 (DSC1) Assay range:30 ng/L -1368 ng/L
klD424Mu 小鼠肌球蛋白ⅤA(MYO5A)ELISA試劑盒 ELISA Kit for Myosin VA (MYO5A) Assay range:30 ng/L -1369 ng/L
klE346Mu 小鼠興奮性氨基酸轉(zhuǎn)運蛋白2(EAAT2)ELISA試劑盒 ELISA Kit for Excitatory Amino Acid Transporter 2 (EAAT2) Assay range:30 ng/L -1370 ng/L
klG368Mu 小鼠鈉/鉀離子轉(zhuǎn)運ATP酶α2肽(ATP1α2)ELISA試劑盒 ELISA Kit for ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) Assay range:30 ng/L -1371 ng/L
klG371Mu 小鼠鈉/鉀離子轉(zhuǎn)運ATP酶β1肽(ATP1β1)ELISA試劑盒 ELISA Kit for ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) Assay range:30 ng/L -1372 ng/L
klG377Mu 小鼠鈣離子轉(zhuǎn)運ATP酶B2(ATP2B2)ELISA試劑盒 ELISA Kit for ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) Assay range:30 ng/L -1373 ng/L
klG916Mu 小鼠泛醌*還原酶核心蛋白Ⅱ(UQCRC2)ELISA試劑盒 ELISA Kit for Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) Assay range:30 ng/L -1374 ng/L
klJ599Mu 小鼠肌動蛋白相關(guān)蛋白2/3復(fù)合體亞基4(ARPC4)ELISA試劑盒 ELISA Kit for Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) Assay range:30 ng/L -1375 ng/L
klC585Mu 小鼠尿皮質(zhì)素2(UCN2)ELISA試劑盒 ELISA Kit for Urocortin 2 (UCN2) Assay range:30 ng/L -1376 ng/L
klD140Mu 小鼠尿皮質(zhì)素3(UCN3)ELISA試劑盒 ELISA Kit for Urocortin 3 (UCN3) Assay range:30 ng/L -1377 ng/L
klE562Mu 小鼠TATA框結(jié)合蛋白關(guān)聯(lián)因子12(TAF12)ELISA試劑盒 ELISA Kit for TATA Box Binding Protein Associated Factor 12 (TAF12) Assay range:30 ng/L -1378 ng/L
klF104Mu 小鼠*/*豐富剪接因子4(SRSF4))ELISA試劑盒 ELISA Kit for Serine/Arginine Rich Splicing Factor 4 (SRSF4) Assay range:30 ng/L -1379 ng/L
klE020Mu 小鼠蛋白磷酸酶3調(diào)節(jié)因子亞基1(PPP3R1)ELISA試劑盒 ELISA Kit for Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Assay range:30 ng/L -1380 ng/L